Modelling effects of phytobiotic administration on coherent responses to Salmonella infection in laying hens

Figures & data

Table 1. Correlation coefficients (r)* between gene expression and blood biochemical/immunological parameters.

Blood parameters	Genes
	AvBD10	IL6	IL8L1	SLC5A1	CA2	CALB1	RARRES1	BPIFB3
Total protein, g/L	−0.173	0.102	−0.225	−0.369	0.183	0.187	−0.175	−0.182
Albumin, g/L	0.228	0.386	0.275	−0.039	−0.135	−0.292	0.068	0.005
Globulins, g/L	−0.242	−0.079	−0.374	−0.333	0.378	0.448	−0.263	−0.128
Urea, mmol/L	−0.531	−0.720	−0.551	−0.574	0.365	0.292	−0.581	−0.242
Urea nitrogen, mmol/L	−0.528	−0.690	−0.547	−0.586	0.398	0.412	−0.588	−0.264
Creatinine, μmol/L	−0.252	−0.332	−0.286	−0.112	0.340	0.205	−0.216	0.055
Alanine aminotransferase, IU/L	0.107	−0.314	0.051	0.030	−0.101	−0.113	0.032	−0.573
Aspartate aminotransferase, IU/L	−0.155	−0.326	0.494	−0.029	−0.126	−0.473	−0.116	0.294
Alkaline phosphatase, IU/L	0.222	0.390	0.010	0.305	−0.214	−0.181	0.330	0.156
Alpha amylase, IU/L	−0.002	0.239	0.508	0.606	−0.361	−0.347	0.505	0.361
Glucose, mmol/L	0.067	0.333	0.752	0.483	−0.552	−0.443	0.421	0.552
Total cholesterol, mmol/L	0.421	0.294	−0.490	−0.296	0.241	0.266	−0.068	−0.395
Calcium, mmol/L	0.107	0.096	−0.560	−0.566	0.291	0.380	−0.326	−0.497
Phosphorus, mmol/L	0.396	0.218	−0.356	−0.324	−0.180	−0.043	−0.051	−0.357
Bilirubin, μmol/L	0.348	0.024	−0.211	0.066	−0.145	−0.148	0.194	−0.509
Uric acid, μmol/L	−0.017	0.390	0.044	−0.181	−0.161	−0.111	−0.048	0.226
Lysozyme activity, %	0.363	0.122	−0.453	−0.209	0.521	0.449	−0.185	−0.377
Bactericidal activity, %	0.216	0.139	0.160	0.158	−0.305	−0.068	0.175	−0.329
Beta-lysine activity, %	0.131	−0.075	0.222	0.037	−0.358	−0.205	−0.012	−0.515
Activity of immunoglobulin IgA	−0.136	−0.296	−0.647	−0.554	0.176	0.261	−0.384	−0.141
IgM	0.423	0.013	−0.453	−0.424	0.349	0.287	−0.257	−0.670
IgG1	0.420	0.392	0.190	0.057	−0.162	−0.207	0.151	0.067
IgG2	0.220	0.408	0.850	0.605	−0.316	−0.506	0.467	0.623

*Pairwise correlation coefficients with |r| > 0.5 are given in bold.

IgM: immunoglobulin IgM; IgG1: immunoglobulin IgG1; IgG2: immunoglobulin IgG2.

Table 2. Accuracy of calculating the relationship of gene expression with biochemical/immunological blood parameters based on their multiple correlation.

Genes	Correlated blood parameters	Calculation accuracy (R^2)	Relationship closeness (R)
AvBD10	U, NU	0.502	0.709
IL6	U, NU	0.538	0.733
IL8L1	U, NU, Am, Gl, Ca, IgA, IgG2	0.855	0.925
SLC5A1	U, NU, Am, Ca, IgA, IgG2	0.556	0.746
CA2	Gl, La	0.518	0.720
CALB1	IgG2	0.290*	0.538*
RARRES1	U, NU, Am	0.688	0.829
BPIFB3	Alt, Gl, Br, Bla, IgM, IgG2	0.758	0.870

U: urea; UN: urea nitrogen; Alt: alanine aminotransferase; Am: alpha amylase; Gl: glucose; Ca: calcium; Br: bilirubin; La: lysozyme activity; Bla: beta-lysine activity; IgA: immunoglobulin IgA; IgM: immunoglobulin IgM; IgG2: immunoglobulin IgG2.

*Single pairwise correlation.