1,545
Views
5
CrossRef citations to date
0
Altmetric
Papers

Preparation and characterisation of monoclonal antibodies against deoxynivalenol

, , , , , , , & show all
Pages 560-568 | Received 17 Nov 2019, Accepted 10 Mar 2020, Published online: 25 May 2020

Figures & data

Figure 1. Molecular structure of DON. DON: deoxynivalenol.

Figure 1. Molecular structure of DON. DON: deoxynivalenol.

Figure 2. The synthetic route of deoxynivalenol artificial antigen. Note: CDI: N,N'-carbonyldiimidazole; BSA: bovine serum albumin.

Figure 2. The synthetic route of deoxynivalenol artificial antigen. Note: CDI: N,N'-carbonyldiimidazole; BSA: bovine serum albumin.

Figure 3. The SDS-PAGE identification of artificial antigen DON-BSA. 1: Control MW, 2: BSA; 3: DON-BSA. Note: SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis; DON-BSA: deoxynivalenol-bovine serum albumin; MW: molecular weight; BSA: bovine serum albumin.

Figure 3. The SDS-PAGE identification of artificial antigen DON-BSA. 1: Control MW, 2: BSA; 3: DON-BSA. Note: SDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresis; DON-BSA: deoxynivalenol-bovine serum albumin; MW: molecular weight; BSA: bovine serum albumin.

Figure 4. The UV identification of artificial antigen DON-BSA. Note: DON-BSA: deoxynivalenol-bovine serum albumin; BSA: bovine serum albumin.

Figure 4. The UV identification of artificial antigen DON-BSA. Note: DON-BSA: deoxynivalenol-bovine serum albumin; BSA: bovine serum albumin.

Figure 5. The IR identification of artificial antigen DON-BSA. Note: DON-BSA: deoxynivalenol-bovine serum albumin; BSA: bovine serum albumin.

Figure 5. The IR identification of artificial antigen DON-BSA. Note: DON-BSA: deoxynivalenol-bovine serum albumin; BSA: bovine serum albumin.

Figure 6. Titre curves of DON pAb determined by indirect ELISA. Note: DON pAb: deoxynivalenol polyclonal antibody; ELISA: enzyme-linked immunosorbent assay.

Figure 6. Titre curves of DON pAb determined by indirect ELISA. Note: DON pAb: deoxynivalenol polyclonal antibody; ELISA: enzyme-linked immunosorbent assay.

Figure 7. Inhibitory curves of DON pAb against DON dermined by blocking ELISA. Note: DON pAb: deoxynivalenol polyclonal antibody; ELISA: enzyme-linked immunosorbent assay.

Figure 7. Inhibitory curves of DON pAb against DON dermined by blocking ELISA. Note: DON pAb: deoxynivalenol polyclonal antibody; ELISA: enzyme-linked immunosorbent assay.

Figure 8. Stability determination of monoclonal cell lines.

Figure 8. Stability determination of monoclonal cell lines.

Figure 9. The inhibition ratio curves of McAb. Note: McAb: monoclonal antibody.

Figure 9. The inhibition ratio curves of McAb. Note: McAb: monoclonal antibody.

Figure 10. The ka curve of DON mAb. Note: DON mAb: deoxynivalenol monoclonal antibody; ka: affinity constant.

Figure 10. The ka curve of DON mAb. Note: DON mAb: deoxynivalenol monoclonal antibody; ka: affinity constant.

Table 1. The percent cross-reactivity of DON mAb with other structural complexes.