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Effect of diet supplemented with enzymatically treated Artemisia annua L. on intestinal digestive function and immunity in weaned pigs

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Pages 1170-1179 | Received 17 May 2020, Accepted 17 Sep 2020, Published online: 06 Oct 2020

Figures & data

Table 1. Composition and nutrient content of basal diet (as-fed basis).

Table 2. Primer sequences of target genes.

Table 3. Effect of diet supplemented with different doses of enzymatically treated Artemisia annua L. on growth performance in weaned pigs at 50 days of age.

Table 4. Effect of diet supplemented with different doses of enzymatically treated Artemisia annua L. on nutrient digestibility in weaned pigs at 50 days of age.

Table 5. Effect of diet supplemented with 2 g/kg enzymatically treated Artemisia annua L. on the development of small intestine in weaned pigs at 51 days of age.

Table 6. Effect of diet supplemented with 2 g/kg enzymatically treated Artemisia annua L. on digestive enzyme activity of intestinal digesta in weaned pigs at 51 days of age.

Table 7. Effect of diet supplemented with 2 g/kg enzymatically treated Artemisia annua L. on disaccharidase, Na+-K+-ATPase and AKP activities of intestinal mucosa in weaned pigs at 51 days of age.

Table 8. Effect of diet supplemented with 2 g/kg enzymatically treated Artemisia annua L. on nutrient transporter function of intestinal mucosa in weaned pigs at 51 days of age.

Figure 1. Effect of diet supplemented with 2 g/kg enzymatically treated Artemisia annua L. on the mRNA expressions of cytokines and immunoglobulin G in the jejunum of weaned pigs at 51 days of age. CON: control group, basal diet; EA3: basal diet + 2 g/kg enzymatically treated Artemisia annua L.; IFN-γ: interferon γ; IL-1β: interleukin 1β; IL-6: interleukin 6; TNF-α: tumour necrosis factor α; IL-4: interleukin 4; IL-10: interleukin 10; IgG: immunoglobulin G. All data were analysed using independent t-test and were presented as mean ± SEM (n = 6). A probability level of p < .05 was considered statistically significant.

Figure 1. Effect of diet supplemented with 2 g/kg enzymatically treated Artemisia annua L. on the mRNA expressions of cytokines and immunoglobulin G in the jejunum of weaned pigs at 51 days of age. CON: control group, basal diet; EA3: basal diet + 2 g/kg enzymatically treated Artemisia annua L.; IFN-γ: interferon γ; IL-1β: interleukin 1β; IL-6: interleukin 6; TNF-α: tumour necrosis factor α; IL-4: interleukin 4; IL-10: interleukin 10; IgG: immunoglobulin G. All data were analysed using independent t-test and were presented as mean ± SEM (n = 6). A probability level of p < .05 was considered statistically significant.

Figure 2. Effect of diet supplemented with 2 g/kg enzymatically treated Artemisia annua L. on the mRNA expressions of cytokines and immunoglobulin G in the ileum of weaned pigs at 51 days of age. CON: control group, basal diet; EA3: basal diet + 2 g/kg enzymatically treated Artemisia annua L; IFN-γ: interferon γ; IL-1β: interleukin 1β; IL-6: interleukin 6; TNF-α: tumour necrosis factor α; IL-4: interleukin 4; IL-10: interleukin 10; IgG: immunoglobulin G. All data were analysed using independent t-test and were presented as mean ± SEM (n = 6). A probability level of p < .05 was considered statistically significant.

Figure 2. Effect of diet supplemented with 2 g/kg enzymatically treated Artemisia annua L. on the mRNA expressions of cytokines and immunoglobulin G in the ileum of weaned pigs at 51 days of age. CON: control group, basal diet; EA3: basal diet + 2 g/kg enzymatically treated Artemisia annua L; IFN-γ: interferon γ; IL-1β: interleukin 1β; IL-6: interleukin 6; TNF-α: tumour necrosis factor α; IL-4: interleukin 4; IL-10: interleukin 10; IgG: immunoglobulin G. All data were analysed using independent t-test and were presented as mean ± SEM (n = 6). A probability level of p < .05 was considered statistically significant.

Table 9. Effect of diet supplemented with 2 g/kg enzymatically treated Artemisia annua L. on immune function of intestinal mucosa in weaned pigs at 51 days of age.