Effects of ammonia-N stress on the antioxidant enzymes, heat shock proteins, and apoptosis-related genes of Macrobrachium rosenbergii

Figures & data

Table 1. Fluorescence quantitative PCR reaction primer.

Gene	Sequence	Genbank
b-actin(F1)	5′-GTGGTCGTGAAGGTGTAGCC-3′	AY626840.1
b-actin(R1)	5′-GAGGGGTATGCACTTCCTCA-3′
CAT(F1)	5′-ACTACAACCAGGAAAGTGCTCCCA-3′	HQ668089.1
CAT(R2)	5′-TGGCGTTCCTCTTCGTTCATGACT-3′
GPX(F1)	5′-AGATGAGCGCAATCAAAAGC-3′	FJ670566.1
GPX(R1)	5′-GCCGTGTTCTCCACAAGAAT-3′
Cu/ZnSOD(F1)	5′-GGAGCTAGATTGGCTTGCTG-3′	MF678811.1
Cu/ZnSOD(R1)	5′-AGAAAATCACTGCCCCTCCT-3′
HSP70(F1)	5′-CCTCTGCCCAAGCAAGTATT-3′	AY466445.1
HSP70(R1)	5′-TACCACGGAACAAGTCACCA-3′
HSP60(F1)	5′-TCTCGGCAAGTAACCACTCC-3′	HG001456.1
HSP60(R1)	5′-CCATCACGACCAACCTTCTC-3′
HSP90(F1)	5′-ATGAACGACGAGACCGAGAC-3′	HG001457.1
HSP90(R1)	5′-GGCATCTGAGGAGTTGGAGA-3′
Caspase3(F1)	5′-AGGGGATAAAACCGATGGAG-3′	KF878972.1
Caspase3(R1)	5′-CCCACATGACGAGCATATCA-3′
Bok(F1)	5′-CGCCACAGTAGGAGAGAAGG-3′	HG530759.1
Bok(R1)	5′-TGAAAACGGCAATGGACATA-3′

Table 2. The LC50 and Sc of ammonia-N stress on M. rosenbergii.

Stress time (h)	Median lethal concentration LC50 (mg·L^−1)	95% Confidence limits	Safe concentration (mg·L^−1)
24	7.72	7.32–8.41	0.378
48	5.44	5.30–5.61
72	4.28	4.19–4.38
96	3.78	3.65–3.95

LC50: Median lethal concentration; Sc: Safe concentration.

Figure 1. Effect of ammonia-N on the T-SOD and CAT activities of haemolymph, muscle and gill of M. rosenbergii. Notes: The different lowercase mean significant difference at same time (p<.05), no lowercase mean no significant differences at same time (p>.05). T-SOD: Total Superoxide Dismutase; CAT: Catalase.

Figure 2. Effect of ammonia-N on the GSH-Px activity of muscle of M. rosenbergii. Notes: The different lowercase mean significant difference at same time (p<.05), no lowercase mean no significant differences at same time (p>.05). GSH-Px/ GPX: Glutathione peroxidase.

Figure 3. Effect of ammonia-N on the MDA content of haemolymph, muscle and gill of M. rosenbergii. Notes: The different lowercase mean significant difference at same time (p<.05), no lowercase mean no significant differences at same time (p>.05). MDA: Malondialdehyde.

Figure 4. Effect of ammonia-N on the relative expression Cu/ZnSOD, CAT, and GPX of muscle and hepatopancreas in M. rosenbergii. Notes: The different lowercase mean significant difference at same time (p<.05), no lowercase mean no significant differences at same time (p>.05). Cu/ZnSOD: Copper and zinc-containing superoxide dismutase; CAT: Catalase; GPX: Glutathione peroxidase.

Figure 5. Effect of ammonia-N on the relative expression of HSP60, HSP70, and HSP90 of muscle and hepatopancreas in M. rosenbergii. Notes: The different lowercase mean significant difference at same time (p<.05), no lowercase mean no significant differences at same time (p>.05).

Figure 6. Effect of ammonia-N on the relative expression of Caspase3 and Bok of muscle and hepatopancreas in M. rosenbergii. Notes: The different lowercase mean significant difference at same time (p<.05), no lowercase mean no significant differences at same time (p>.05).