Hepatic expression responses of DNA methyltransferases, heat shock proteins, antioxidant enzymes, and NADPH 4 to early life thermal conditioning in broiler chickens

Figures & data

Table 1. A list of the primer’s sequences and annealing temperature (Tm) used in the present study.

Gene	Primer sequence (5`-3`)		Accession number	Annealing Tm (°C)
hsp70	F	GATCTGGGCACCACGTATTCT	FJ217667.1	58
	R	GGTTCATTGCCACTTGGTTCTT
hsp90A	F	ACA CAT GCC AAC CGC ATT TA	NM_001109785.1	57
	R	CCT CCT CAG CAG CAG TAT CA
hsp90B	F	AGT AGA GAA GGG TCC CGA AC	NM_204289.1	57
	R	GGA TGC ATT TAG GCC ATC CA
hsp60	F	CGCAGACATGCTCCGTTTG	NM_001012916	56
	R	TCTGGACACCGGCCTGAT
hspA9	F	GGC ATG ACC AGA ATG CCA AAG	NM_001006147.1	56
	R	TTG TGA AGA CAC CGC CTA ATG T
GR	F	GCCATCGTGAAAAGAGAAGG	NM_001037826	54
	R	TTTCAACCACATCGTGCAT
SOD	F	AGG GGG TCA TCC ACT TCC	NM_205064.1	54
	R	CCC ATT TGT GTT GTC TCC AA
SOD2	F	CTG ACC TGC CTT ACG ACT ATG	NM_204211.1	56
	R	CGC CTC TTT GTA TTT CTC CTC T
CAT	F	GAA GCA GAG AGG TTC CCA TTT A	NM_001031215.2	56
	R	CAT ACG CCA TCT GTT CTA CCT C
NOX4	F	CCA GAC CAA CTT AGA GGA ACA C	NM_001101829.1	55
	R	TCT GGG AAA GGC TCA GTA GTA
DNMT1	F	CATCCTCAGGGACCACATCT	NM_206952	58
	R	CTTCTTCTCGTGGTGGGTGT
DMAP1	F	CTTTGTGCTGCCTACTGCTG	XM_004936716	58
	R	AGTTCTGCTCCTTGCTTTGC
DNMT3A	F	CACCACTCGCTCCAACTCCA	NM_00102483223	60
	R	ATGTTGGACACGTCCGTGT
DNMT3B	F	CTGCACACAGAGCTCGCTAC	NM_00102482833	60
	R	GAGAGGGGAGAAGAGGTGCT
β-actin	F	CACAATGTACCCTGGCATTG	L08165	54-56
	R	ACATCTGCTGGAAGGTGGAC
GAPDH	F	CTTTGGCATTGTGGAGGGTC	NM_204305	58-60
	R	ACGCTGGGATGATGTTCTGG

Table 2. Effect of thermal conditioning (TC) on plasma corticosterone (CORT) levels (ng/mL) of broiler chicks.

Experimental groups	Post-conditioning response
	Control	Thermal conditioned
At hatch	46.11^C	–
TC3	35.64^D	72.32^A
TC5	33.79^D	45.61^C
TC7	17.89^E	57.34^B
	Five weeks old
CE	18.30^b
TCE3	27.98^a
TCE5	8.45^d
TCE7	12.50^c

TC3: thermal conditioning on 3^rd day of age (DOA); TC5: thermal conditioning on 5^th DOA; TC7: thermal conditioning on 7^th DOA; CE: control exposed at 35 DOA; TCE3: 3-day conditioned exposed at 35 DOA; TCE5: 5-day conditioned exposed at 35 DOA; TCE7: 7-day conditioned exposed at 35 DOA.

^a,b,c,dMeans in the same column with different superscripts are significantly different at (p ≤ .05).

Figure 1. Effect of thermal conditioning (TC) on mRNA expression of hsp70, hsp60, hsp90B and hsp90A in the liver of broiler chicks. Con.0: Control post-hatch; C3: control on 3 day of age (DOA); TC3: thermal conditioning on 3 DOA; C5: control on 5 DOA; TC5: thermal conditioning on 5 DOA; C7: control on 7 DOA; TC7: thermal conditioning on 7 DOA; CE: control at 35 DOA; TCE3: 3-day conditioned exposed at 35 DOA; TCE5: 5-day conditioned exposed at 35 DOA; TCE7: 7-day conditioned exposed at 35 DOA.

Figure 2. Effect of thermal conditioning (TC) on mRNA expression of hspA9, SOD2 and NOX4 in the liver of broiler chicks. Con.0: Control post-hatch; C3: control on 3 DOA; TC3: thermal conditioning on 3 DOA; C5: control on 5 DOA; TC5: thermal conditioning on 5 DOA; C7: control on 7 DOA; TC7: thermal conditioning on 7 DOA; CE: control at 35 DOA; TCE3: 3-day conditioned exposed at 35 DOA; TCE5: 5-day conditioned exposed at 35 DOA; TCE7: 7-day conditioned exposed at 35 DOA.

Figure 3. Effect of thermal conditioning (TC) on mRNA expression of CAT and SOD in the liver of broiler chicks. Con.0: Control post-hatch; C3: control on 3 DOA; TC3: thermal conditioning on 3 DOA; C5: control on 5 DOA; TC5: thermal conditioning on 5 DOA; C7: control on 7 DOA; TC7: thermal conditioning on 7 DOA; CE: control at 35 DOA; TCE3: 3-day conditioned exposed at 35 DOA; TCE5: 5-day conditioned exposed at 35 DOA; TCE7: 7-day conditioned exposed at 35 DOA.