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Research Papers

CWD prions remain infectious after passage through the digestive system of coyotes (Canis latrans)

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Pages 367-375 | Received 07 Jul 2015, Accepted 18 Aug 2015, Published online: 21 Dec 2015

Figures & data

TABLE 1. Coyote number, sex, age and treatment group

TABLE 2. Transgenic mouse bioassay results. The number of mice that died from CWD/total number of mice intracerebrally inoculated per group with coyote feces. Pre-ingestion indicates fecal samples collected prior to oral ingestion of CWD-positive elk brain homogenate, and days 1, 2, and 3 after ingestion. Day 1 post-ingestion resulted in 23% of the mice becoming terminally ill, day 2, 38% and day 3, 38%

Figure 1. Coyote lymph node immunohistochemistry. Images are a representation of findings. (A) CWD-positive control elk retropharyngeal lymph node. Control coyote (B), and treatment coyote (C), retropharyngeal lymph node. 20X magnification.

Figure 1. Coyote lymph node immunohistochemistry. Images are a representation of findings. (A) CWD-positive control elk retropharyngeal lymph node. Control coyote (B), and treatment coyote (C), retropharyngeal lymph node. 20X magnification.

Figure 2. Transgenic mouse bioassay of coyote feces. All control mice and mice inoculated with feces collected prior to ingestion of CWD-positive elk brain remained disease-free for the duration of the study. Deaths occurred in all of the DPI tested, however, disease penetrance was incomplete. Mice inoculated with DPI 3 feces lived slightly longer than DPI 1 and 2. Each DPI group represented above combines survival times of mice from each of the study coyotes.

Figure 2. Transgenic mouse bioassay of coyote feces. All control mice and mice inoculated with feces collected prior to ingestion of CWD-positive elk brain remained disease-free for the duration of the study. Deaths occurred in all of the DPI tested, however, disease penetrance was incomplete. Mice inoculated with DPI 3 feces lived slightly longer than DPI 1 and 2. Each DPI group represented above combines survival times of mice from each of the study coyotes.