Detection of a new 20-bp insertion/deletion (indel) within sheep PRND gene using mathematical expectation (ME) method

Figures & data

FIGURE 1. Electrophoresis pattern of the novel indel variants of sheep PRND gene.

TABLE 1. Diversity parameters for the novel 20-bp indel of sheep PRND gene.

	Sizes	Genotypic frequencies			Allelic frequencies		^aHWE	Population parameters
Breeds	N	DD	ID	II	D	I	P values	Ho	He	Ne	PIC
LFTS	61	0.000	0.016	0.984	0.008	0.992	P > 0.05	0.984	0.016	1.016	0.016
STHS	196	0.031	0.107	0.862	0.084	0.916	P < 0.05	0.846	0.154	1.182	0.142
TS	166	0.018	0.006	0.976	0.021	0.979	P < 0.05	0.959	0.041	1.043	0.040
HS	200	0.025	0.115	0.860	0.083	0.917	P < 0.05	0.849	0.151	1.178	0.140

Note:

^a HWE, Hardy-Weinberg equilibrium; Ho, homozygosity; He, heterozygosity; Ne, effective allele numbers; PIC, Polymorphism information content; LFTS, Lanzhou Fat-Tail sheep; STHS, Small Tail Han sheep; TS, Tong sheep; HS, Hu sheep

TABLE 2. All reaction times of different group in 4 sheep breeds.

Breeds	LFTS	STHS	TS	HS
Sizes	61	196	166	200
MAF	0.01	0.08	0.02	0.08
NR1	1	1	1	1
RT1	61	196	166	200
NGn	10	4	7	4
pRTn	12	105	46	107
RTn	11	125	52	126
RR	83.33%	36.22%	68.67%	37.00%

Note: MAF, minor allelic frequencies; NR_n, number of individuals in one reaction time; NG_n, the optimal number of individuals in one mixed group; RT_n, reaction times; pRT_n, predicting treaction times by the formulate; RR, reduction rate.

FIGURE 2. Sequence diagram of the 20-bp indel variants of sheep PRND gene.

TABLE 3. Relationship between the 20-bp indel locus of PRND gene and growth related traits in Hu sheep (LSM^a ± SE) (P < 0.05).

		Observed genotypes (LSM^a ± SE)
Breeds	Growth traits	DD	ID	II	p values
HS	CaCI	^b11.07 ± 0.15	^a11.79 ± 0.14	^ab11.49 ± 0.07	0.012
	TI	^b105.37 ± 1.74	^a111.49 ± 1.44	^ab108.08 ± 0.50	0.037
	HW(cm)	18.30 ± 0.49	17.09 ± 0.22	17.43 ± 0.08	0.070
	ChCI	118.20 ± 1.69	126.88 ± 1.66	124.34 ± 0.63	0.082