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Research Paper

Polymorphism of prion protein gene (PRNP) in Nigerian sheep

Adeniyi C. Adeolaa State Key Laboratory of Genetic Resources and Evolution & Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China;b Sino-Africa Joint Research Center, Chinese Academy of Sciences, Kunming, China;c Centre for Biotechnology Research, Bayero University, Kano, NigeriaCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0001-5171-7878View further author information
ORCID Icon,
Semiu F. Bellod Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, ChinaView further author information
,
Abdussamad M. Abdussamadc Centre for Biotechnology Research, Bayero University, Kano, Nigeria;e Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Bayero University, Kano, NigeriaView further author information
,
Akanbi I. Markf Ministry of Agriculture and Rural Development, Secretariat, Ibadan, NigeriaView further author information
,
Oscar J. Sankeg Taraba State Ministry of Agriculture and Natural Resources, Jalingo, NigeriaView further author information
,
Anyebe B. Onojah Department of Virology, College of Medicine, University of Ibadan, Ibadan, NigeriaView further author information
,
Lotanna M. Nnejii Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USAView further author information
,
Nasiru Abdullahij Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University, Kano, NigeriaView further author information
,
Sunday C. Olaogunk Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, NigeriaView further author information
,
Lawal D. Rogol Department of Medical Laboratory Science, Faculty of Allied Health Sciences, College of Health Sciences, Bayero University, Kano, NigeriaView further author information
,
Godwin F. Mangbonm Division of Veterinary Office, Serti, NigeriaView further author information
,
Shamsudeen L. Pedroc Centre for Biotechnology Research, Bayero University, Kano, NigeriaView further author information
,
Manasseh P. Hiinann Small Ruminant Section, Solomon Kesinton Agro-Allied Limited Iperu-Remo, Ogun State, NigeriaView further author information
,
Muhammad M. Mukhtarj Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University, Kano, NigeriaView further author information
,
Jebi Ibrahimo Department of Veterinary Surgery and Theriogenology, College of Veterinary Medicine, University of Agriculture Makurdi, Makurdi, NigeriaView further author information
,
Hayatu Saidul Department of Medical Laboratory Science, Faculty of Allied Health Sciences, College of Health Sciences, Bayero University, Kano, NigeriaView further author information
,
Philip M. Dawudap Department of Animal Science, Faculty of Agriculture, National University of Lesotho, Lesotho, Southern AfricaView further author information
,
Rukayya K. Balac Centre for Biotechnology Research, Bayero University, Kano, NigeriaView further author information
,
Hadiza L. Abdullahie Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, Bayero University, Kano, NigeriaView further author information
,
Adebowale E. Salakoq Department of Animal Science, Faculty of Agriculture, University of Ibadan, Ibadan, NigeriaView further author information
,
Samia Kdidir Livestock and Wildlife Laboratory, Institut des Régions Arides, Université de Gabes, Medenine, TunisiaView further author information
,
Mohamed Habib Yahyaouir Livestock and Wildlife Laboratory, Institut des Régions Arides, Université de Gabes, Medenine, TunisiaView further author information
&
Ting-Ting Yina State Key Laboratory of Genetic Resources and Evolution & Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, ChinaView further author information
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Pages 44-54 | Received 28 Sep 2022, Accepted 15 Nov 2022, Published online: 09 Mar 2023

Figures & data

Table 1. Genotype and allele frequencies of 19 PRNP polymorphism in Nigerian sheep.

Table 2. Linkage Disequilibrium (LD) of nineteen (19) PRNP polymorphisms in Nigerian sheep.

Table 3. Haplotype frequencies of fourteen (14) non-synonymous single nucleotide polymorphism of PRNP gene in Nigerian sheep.

Figure 1. Comparisons of the allele frequencies of PRNP codons 154 and 240 in Central and Southern Italy, Chinese and Nigerian sheep. (a) Comparisons of the allele frequency of the PRNP codon 154 between Central and Southern Italy, Chinese sheep and Nigerian sheep [Citation30,Citation63] (b) Comparisons of the allele frequency of PRNP codon 240 between Chinese and Nigerian sheep [Citation30].

Figure 1. Comparisons of the allele frequencies of PRNP codons 154 and 240 in Central and Southern Italy, Chinese and Nigerian sheep. (a) Comparisons of the allele frequency of the PRNP codon 154 between Central and Southern Italy, Chinese sheep and Nigerian sheep [Citation30,Citation63] (b) Comparisons of the allele frequency of PRNP codon 240 between Chinese and Nigerian sheep [Citation30].

Table 4. Distributions of haplotype and genotype frequencies at PRNP codons 143, 154 and 171 between scrapie affected and healthy sheep.

Figure 2. Prediction of amyloid propensity of sheep prion protein according to nonsynonymous SNPs. AMYCO predicted amyloid propensity as values from 0.0 to 1.0. The AMYCO scores<0.45 and>0.78 indicated low and high aggregation propensities of the protein, respectively. ‘HYNN’ indicates haplotype of serine allele at the codon 139, serine allele at the codon 146, histidine allele at the codon 154, and Isoleucine allele at the codon 193. ‘HYKK’ indicates haplotype of Arginine allele at the codon 139, asparagine allele at the codon 146, arginine allele at the codon 154, and threonine allele at the codon 193. ‘HDKK’ indicates haplotype of arginine allele at the codon 139, asparagine allele at the codon 146, histidine allele at the codon 154, and threonine allele at the codon 193. ‘RYNN’ indicates haplotype of arginine allele at the codon 139, asparagine allele at the codon 146, arginine allele at the codon 154, and isoleucine allele at the codon 193.

Figure 2. Prediction of amyloid propensity of sheep prion protein according to nonsynonymous SNPs. AMYCO predicted amyloid propensity as values from 0.0 to 1.0. The AMYCO scores<0.45 and>0.78 indicated low and high aggregation propensities of the protein, respectively. ‘HYNN’ indicates haplotype of serine allele at the codon 139, serine allele at the codon 146, histidine allele at the codon 154, and Isoleucine allele at the codon 193. ‘HYKK’ indicates haplotype of Arginine allele at the codon 139, asparagine allele at the codon 146, arginine allele at the codon 154, and threonine allele at the codon 193. ‘HDKK’ indicates haplotype of arginine allele at the codon 139, asparagine allele at the codon 146, histidine allele at the codon 154, and threonine allele at the codon 193. ‘RYNN’ indicates haplotype of arginine allele at the codon 139, asparagine allele at the codon 146, arginine allele at the codon 154, and isoleucine allele at the codon 193.

Table 5. Measurement of the effect of amino-acid substitutions of PRNP nonsynonymous SNPs in Nigerian sheep.

Supplemental material

Supplemental Material

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Data availability statement

Accession no. MZ463500 – MZ463625 https://www.ncbi.nlm.nih.gov/

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