Estimating sequence diversity of prion protein gene (PRNP) in Portuguese populations of two cervid species: red deer and fallow deer

Figures & data

Figure 1. Map of the east centre of Portugal (Beira Baixa district) showing the geographic locations where different samples were obtained.

Table 1. Amino acid variation within the exon 3 and haplotype frequencies of cervid PRNP in red deer populations.

	Total			Termas		Rosmaninho		Vale Feitoso
Genotypes	Number	Frequency		Frequency		Frequency		Frequency
T98-Q226	81	71.05	16	53.33	42	67.74	30	55.56
T98-E226	29	25.44	12	40.00	16	25.81	22	40.74
A98-Q226	4	3.51	2	6.67	4	6.45	2	3.70
Total	114	100.00	30	100.00	62	100.00	54	100.00

Table 2. Genotype frequencies of PRNP polymorphisms in Portuguese red deer populations.

Acc/Gcc	Cag/Gag
T/A	Q/E		Termas			Rosmaninho		Vale Feitoso
98	226		Number	Frequency	Number	Frequency	Number	Frequency	Number	Frequency
TT	QQ	TQ/TQ	30	52.63	3	33.33	17	62.96	8	42.11
TT	QE	TQ/TE	19	33.33	5	55.56	7	25.93	7	36.84
TT	EE	TE/TE	4	7.02		0.00	1	3.70	3	15.79
TA	QQ	TQ/AQ	2	3.51		0.00	2	7.41		0.00
TA	QE	TE/AQ	2	3.51	1	11.11		0.00	1	5.26
		Total	57	100.00	9	100.00	27	100.00	19	100.00

Figure 2. Protein sequence alignment of new and old world cervid showing conserved homology between species and other molecular conserved features.