Figures & data
Figure 1. Neutralizing IL-13 stimulated the recovery of ECs after balloon injury. (a) schematic summary of the balloon injury model. (b) ELISA analysis of IL-13 serum level. **p < .01 vs. normal; ##p < .01 vs. injury. (c) treatment with IL-13Nab (30 ng) inhibited intimal hyperplasia following balloon injury after 14 days. ##p < .01 vs. injury. Scale bars: 200 μm. (d-e) silver andEvans blue staining to detect the recovery of endothelial cells after balloon injury. (f) immunofluorescence staining for CD31 (red) and DAPI (blue), scale bars: 200 μm. The results were derived from 3 independent experiments each performed in duplicate. Normal, nor. Injury, Inj. Neutralizing IL-13, IL-13Nab.
Figure 2. rhIL-13 inhibited HUVECs migration. (a) rhIL-13 (from 12.5 to 100 ng/mL) did not affect the viability of cultured HUVECs in either the presence or absence of serum after 24 h, as measured by MTT. ***p < .001 vs. normal. (b-c) rhIL-13 (100 ng/mL) did not stimulate the proliferation of HUVECs. Scale bars: 200 μm. (d) the co-application of IL-13 Rα1 Fc (4 μg/mL) and rhIL-13 inhibited the rhIL-13 induced inhibition of cell migration in cultured HUVECs. **p < .01 vs. Ctrl; ##p < .01 vs. rhIL-13. Scale bars: 200 μm. The results were derived from 3 independent experiments each performed in duplicate. Data represent the mean±SD. Control, Ctrl.
Figure 3. rhIL-13 inhibited HUVECs migration via the JAK-1/STAT-3 signaling pathway. (a) rhIL-13 regulated the activation of the JAK-1/STAT-3 signaling pathway. **p < .01 vs. Ctrl; ##p < .01 vs. rhIL-13. (b) the co-application of stattic and rhIL-13 inhibited the rhIL-13 induced inhibition of cell migration in cultured HUVECs. **p < .01 vs. Ctrl; ##p < .01 vs. rhIL-13. Scale bars: 200 μm. (c) STAT-3 inhibition eliminated the accumulation of ROS induced by rhIL-13. **p < .01 vs. Ctrl; ##p < .01 vs. rhIL-13. Scale bars: 200 μm. The results were derived from 3 independent experiments each performed in duplicate. Data represent the mean±SD. Control, Ctrl. Negative control, NC. Ruxolitinib, Rux. Stattic, Stt.
Figure 4. rhIL-13 stimulated ROS accumulation via the NOX-4. (a) rhIL-13 stimulated the expression of NOX-4. **p < .01 vs. Ctrl; ##p < .01 vs. rhIL-13. (b) transfection efficiency of NOX-4 siRNA in cultured HUVECs. **p < .01 vs. Ctrl. (c) NOX-4 inhibition eliminated the accumulation of ROS induced by rhIL-13. **p < .01 vs. Ctrl; ##p < .01 vs. rhIL-13. Scale bars: 200 μm. (d) the co-application of NOX-4 siRNA and rhIL-13 inhibited the rhIL-13 induced inhibition of cell migration in cultured HUVECs. **p < .01 vs. Ctrl; ##p < .01 vs. rhIL-13. Scale bars: 200 μm. (e) F-actin staining after treatment with rhIL-13. Scale bars: 200 μm. The results were derived from 3 independent experiments each performed in duplicate. Data represent the mean±SD. Control, Ctrl. Negative control, NC.
Data availability statement
The data used to support the findings of this study are available from the corresponding author upon request