Figures & data
Figure 1. Lin-28 levels and subcellular distribution are nutrient sensitive. (A) LIN-28::Venus expression in the intestinal progenitor cells of fed (left) and starved (right) animals. Insets are magnified views. LIN-28::Venus was detected with anti-GFP antibodies. (B) LIN-28 expression in the intestinal progenitor cells of fed (left) and starved (right) animals. LIN-28::Venus was detected with anti-LIN-28 antibodies. (C) Scatter dot plot showing the area of Lin-28::Venus expressing progenitor cells in fed vs. starved animals. Mean and standard deviation (SD) are shown. (D) Scatter dot plot of the Corrected Total Cell Fluorescence (CTCF) of Lin-28::Venus expressing cells in fed vs. starved animals. Mean and SD are shown. (E) Scatter dot plot of the CTCF readings from Lin-28 expressing cells in fed vs. starved animals. Mean and SD are shown.
Figure 2. Nutrient-dependent increase in Lin-28::Venus does not require RNA-binding Activity. (A-D) Lin-28::Venus, Lin-28::VenusCSD-, Lin-28::VenusCCHC-, and Lin-28::VenusCSD- CCHC- expression in intestinal progenitor cells from fed (A-D) and starved (A'-D’) animals, respectively. Insets in C' and D’ are magnified views of a starved intestinal progenitor cell stained for Lin-28::Venus (left) and DAPI (right).
