Figures & data
Figure 1. Exposing flies to DCV by placing them in DCV-contaminated vials for 3 d resulted in flies acquiring replicating virus as shown by the increase in DCV titres over time (1A). Gray points show the expression of DCV RNA titres relative to the expression of rp49, an internal fly control gene; black bars are mean titres (B). This orally acquired DCV infection had a moderate effect on fly survival in both male (full circle) and female (full triangle) flies compared to uninfected control male (open circle) and female (open triangle) flies (dashed lines). Data are means ± SEM.
Figure 2. Single-sex groups of flies that had been previously exposed either to DCV or to a sterile Ringers solution were tested in a ‘no-risk’ environment (choice between 2 clean vials; light gray) or a ‘high-risk’ environment (choice between a clean vial and a DCV-contaminated vial; black). The motivation to seek out a food source, measured as the proportion of flies in the cage that landed on any of the provided food sources, increased over time (A). (B) shows the average motivation across the whole observation period for each combination of fly sex, prior DCV exposure and current exposure risk (‘no-risk’ environment (light gray) or a ‘high-risk’ environment (black). Data show means ± SEM.
Figure 3. The proportion of flies in the high-risk cage that preferred to settle on the clean food source over the DCV-contaminated food source, according to sex and previous DCV exposure. Data are means ± SEM.
Figure 4. Schematic of the experimental setup.
