RIS-1/psoriasin expression in epithelial skin cells indicates their selective role in innate immunity and in inflammatory skin diseases including acne

Figures & data

Figure 1. rtPCR detection of CYP26AI, CRABP-II and RIS-1/psoriasin mRNA expression. Lanes 1–3, primary human keratinocytes; lanes 4–6, primary human melanocytes; lanes 7–9, primary human fibroblasts, lane 10, DNA mass ladder; lanes 11–13, human immortalized vascular endothelial cell line HMEC-1; lanes 14–16, human promyelocytic leukemia cell line HL-60, lanes 17–19, human immortalized SZ95 sebocytes; lane 20, DNA mass ladder. DMSO control (C, lanes 1, 4, 7, 11, 14, 17), 13cis-retinoic acid in DMSO (13cRA, 10⁻⁷ M; lanes 2, 5, 8, 13, 16, 19), all-trans retinoic acid in DMSO (atRA, 10⁻⁷ M; lanes 3, 6, 9, 12, 15, 18).

Figure 2. Northern blot detection of RIS-1/psoriasin mRNA levels. Lanes 1–3, primary human keratinocytes; lanes 4–6, human immortalized SZ95 sebocytes; lanes 7–11, primary human fibroblasts, lane 12, human prostatic carcinoma LNCaP cell line; lane 13, RNA mass ladder. DMSO control (C; lanes 1, 4, 7, 13), all-trans retinoic acid in DMSO (atRA, 10⁻⁷ M; lanes 2, 3, 5, 6, 10, 11), 13cis-retinoic acid in DMSO (13cRA, 10⁻⁷ M; lanes 8, 9).

Figure 3. Detection of cellular compartmentation of RIS-1/psoriasin mRNA expression by in situ hybridization. A) Normal skin (40x), B) normal sebaceous glands (100x), C) acne skin (100x), d) psoriatic skin (40x). (1) Stratum granulosum; (2), follicular outer root sheath; (3) follicular inner root sheath; (4) sebaceous gland.

Table 1. Primers used for rtPCR analysis.

Gene	Sense primer location	Anti-sense primer location	PCR product (bp)
CYP26AI	AGG CAC TAA AGC AAT CTT CAA	CAT GGA AAT GGG TGA ATC TTG	621
CRABP-II	CAACTGGAAAATCA-TCCGA	CAACGTCATCCGC[C/T]GTCAT	413
RIS-1/psoriasin	TTAC-CTCGCCGACGTCTTTG	TCACTGGCTGCCC-CCGGAA	246
β-actin	ACCCACACTGTGCCCATCTA	CGGAACCGCTCATTGCC	290