Cytogenetic Analysis of In Vivo and In Vitro Matured Oocytes Derived from Naturally Cycling and Stimulated Mice

Figures & data

Comparison of the Length of Meiotic Spindle Plate of In Vivo and In Vitro Matured Oocytes Derived from Naturally Cycling or Gonadotropin Stimulated Mice (5 Replicates)*

Group	No. of oocytes examined	No. of oocytes with meiotic spindle plate (%)	Length of meiotic spindle plate (μm±SD)
Natural, post-ovulation (in vivo)	45	41 (91.1)	4.7±1.1
Natural, pre-ovulation (in vitro)	51	46 (90.2)	5.0±1.1
Stimulation, post-ovulation (in vivo)	46	41 (89.1)	4.8±1.4
Stimulation, pre-ovulation (in vitro)	54	49 (90.7)	5.1±0.8

*There were no significant differences among groups.

Figure 1 Measurement for the length of spindle plate of mouse oocyte using the PolScope imaging system. The length of spindle plate was measured by crossing the chromosomal alignment (arrow). Scale bar indicates 10 μm.

Comparison of Meiotic Spindle Organization and Chromosomal Alignment of In Vivo and In Vitro Matured Oocytes Derived from Naturally Cycling or Gonadotropin Stimulated mice (10 Replicates)*

		Meiotic spindle and chromosomal alignment (%)
Group	No. of oocytes examined	Normal	Abnormal
Natural, post-ovulation (in vivo)	110	108 (98.2)	2 (1.8)
Natural, pre-ovulation (in vitro)	104	100 (96.2)	4 (3.8)
Stimulation, post-ovulation (in vivo)	112	106 (94.6)	6 (5.4)
Stimulation, pre-ovulation (in vitro)	105	101 (96.2)	4 (3.8)

*There were no significant differences among groups.

Figure 2 Morphology of meiotic spindle organization and chromosome alignment in normal and abnormal mouse oocytes. (A) Oocyte with normal meiotic spindle organization (green); (a) The same oocyte with normal chromosome alignment (blue); (B) Oocyte with abnormal meiotic spindle organization (green); (b) The same oocyte with abnormal chromosome alignment (blue). Scale bar indicates 20 μm.

Figure 3 Normal (haploidy) chromosomes in parthenogenetically activated mouse oocyte. The oocyte with 20 chromosomes. Scale bar indicates 20 μm.

Ploidy Analysis of In Vivo and In Vitro Matured Oocytes Derived from Naturally Cycling or Gonadotropin Stimulated Mice (6 Replicates)*

		Ploidy (%)
Group	No. of oocytes examined	Haploidy (n=20)	Aneuploidy (n > or < 20)
Natural, post-ovulation (in vivo)	50	47 (94.0)	3 (6.0)
Natural, pre-ovulation (in vitro)	48	45 (93.8)	3 (6.2)
Stimulation, post-ovulation (in vivo)	51	46 (90.2)	5 (9.8)
Stimulation, pre-ovulation (in vitro)	52	47 (90.4)	5 (9.6)

*There were no significant differences among groups.