Antibodies Against Laminin-1 and Sperm, Intraacrosomal Proteins in Semen from Infertile Couples

Figures & data

FIGURE 1 Levels of a-ln-1 associated with semen pathology. Results of analysis of semen a-Ln-1- antibodies against laminin-1, increased cellularity >5×10⁶ round cells/ml (macrophages, leucocytes or/and lymphocytes), detection of sperm agglutinating antibodies by direct mixed antiimunoglobulin reaction test (MAR) in IgG, IgA, IgM, and IgE, intraacrosomal proteins exemined by monoclonal antiboides Hs8 (IgG), Hs-14 (IgM) and Hs-36 (IgM).

Results of Analysis of Semen

	Pathology with higher a-Ln-1 levels	Pathology without higher a-Ln-1 levels	Normospermatics
Mean age	33.5	31.9	35.3
Number of samples	27/61 (44%)	34	10
Increased cellularity in semen	18/27 (67%)	15/34 (44%)	5/10 (50%)
MAR-test
IgG	4/27 (14.8%)	6/34 (17.6%)	3/10 (30%)
IgA	6/27 (22.2%)	8/34 (23.5%)	2/10 (20%)
IgM	1/27 (3.7%)	1/34 (2.9%)	0
IgE	0	3/34	(9%)
Intraacrosomal proteins positivity
Hs8 (IgG)	0	0	9/10 (90%)
Hs14 (IgM)	0	1/34 (3%)	10/10 (100%)
Hs36 (IgM)	1/27 (4%)	0	9/10 (90%)
Head of the sperm pathology	3/27 (11%)	3/34 (9%)	0

Analysis of semen by a-Ln-1- (antibodies against laminin-1). Increased cellularity means more than 5×10⁶ round cells/ml of macrophages, leucocytes or/and lymphocytes. Detection of sperm agglutinating antibodies by direct mixed antiimunoglobulin reaction test (MAR) in IgG, IgA, IgM, and IgE. Intraacrosomal proteins examined by monoclonal antiboides Hs8 (IgG), Hs-14 (IgM) and Hs-36 (IgM).