Impact of hydrosalpinx fluid on early human embryos

Figures & data

Table 1. Comparison of chemical components between the two hydrosalpinx fluid (HF) groups and the blastocyst culture medium.

Index	Colored HF (n=16)	Colorless HF (n=17)	Blastocyst culture medium (n=9)	P
pH	7.53±0.08**	7.55±0.09**	7.42±0.08	< 0.05
Osmotic pressure (mOsm/kg)	265.48±10.25	260.35±7.24	271.05±16.24	NS
K^+ (mmol/L)	3.75±0.09**	3.64±0.82**	5.80±0.72	< 0.05
Na^+ (mmol/L)	142.32.±8.24	135.09±8.22	144.32±7.34	NS
Cl^− (mmol/L)	118.85±9.05	115.2±7.86	122.75±8.25	NS
CO2 (mmol/L)	26.51±9.24**	29.67±10.21**	19.95±7.24	< 0.01
Ca^2+ (mmol/L)	1.55±0.12**	1.54±0.44**	1.85±0.16	< 0.05
Mg^2+ (mmol/L)	0.31±0.02**	0.32±0.03**	1.97±0.52	< 0.01
P (mmol/L)	-	-	0.26±0.03	< 0.01
Total proteins (g/L)	-	-	-
Glucose (mmol/L)	0.14±0.03*	0.28±0.05**	3.39±0.03	< 0.01

Table 2. Comparison of cytokines and microbiological tests between the two HF groups.

Index	Colored HF (n=16)	Colorless HF (n=17)	P
TNF-ɑ (pg/ml)	32.7±4.52	23.40±4.09	< 0.05
IL-2 (pg/ml)	29.9±4.55	20.35±4.24	< 0.05
Chlamydia antigen-positive rate (%)	62.5 (10/16)	70.6 (12/17)	NS
Bacterial culture-positive (%)	0	0	NS

HF: hydrosalpinx fluid.

Table 3. In vitro impacts of hydrosalpinx fluid (HF) with different concentrations and colors on embryos.

Group	Number of D3 embryos	Blastulation rate (%)	Blastocyst hatching rate (%)	High-quality blastocyst rate (%)
0% HF	166	19.9 (33/166)*	51.5 (17/33)*	72.7 (24/33)*
25% HF	165	19.4 (32/165)*	53.1 (17/32)*	75.0 (24/32)*
50% HF	166	20.5 (34/166)*	52.9 (18/34)*	73.5 (25/34)*
75% HF	163	12.3 (20/163)	25.0 (5/20)	20.0 (4/20)
Colorless HF	255	17.6 (45/255)	48.9 (22/45)	64.4 (29/45)
Colored HF	239	17.2 (41/239)	43.9 (18/41)	58.5 (24/41)

* P < 0.05, compared with 75% HF.