Evaluation of the impact of sperm morphology on embryo aneuploidy rates in a donor oocyte program

Figures & data

Table 1. Descriptive statistics in groups.

	Kruger’s Score Group
	1 (n = 13)	2 (n = 20)	3 (n = 32)	4 (n = 16)	5 (n = 22)
	Mean±SD	Mean±SD	Mean±SD	Mean±SD	Mean±SD	p
Paternal Age	41.3 ± 5.7	43.8 ± 6.5	42.6 ± 5.1	43.9 ± 5.5	42.8 ± 5.6	0.878
Maternal Age	42.5 ± 3.7	43.2 ± 4.8	43.2 ± 4.4	42.1 ± 5.8	41.3 ± 4.7	0.873
Donor Age	23.4 ± 2.8	21.8 ± 1.5	25.1 ± 1.7	23.6 ± 1.9	24.2 ± 2.0	0.871
Sperm Count/ml	60.1 ± 44.2	52.8 ± 40.4	51.2 ± 34.5	58.1 ± 35.9	68.5 ± 52.4	0.782
Total Motility	55.9 ± 15.0	46.7 ± 20.9	42.4 ± 19.3	50.3 ± 15.1	51.6 ± 16.3	0.124
Percentage of Aneuploidy	62.2 ± 18.9	56.7 ± 19.5	39.6 ± 12.5	41.4 ± 14.7	27.0 ± 11.5	<0.001^a
Percentage of Trisomy	19.2 ± 13.8	19.2 ± 13.2	12.6 ± 10.4	13.4 ± 11.1	9.0 ± 9.5	0.042^a
Percentage of Monosomy	24.7 ± 13.2	22.2 ± 16.2	16.8 ± 11.7	16.5 ± 10.0	9.8 ± 9.6	0.004^a
Percentage of Normal Embryo	30.5 ± 18.7	40.9 ± 20.3	56.2 ± 14.2	54.3 ± 16.4	71.2 ± 13.0	<0.001^a
Fertilization	70.1 ± 16.8	74.6 ± 16.9	73.7 ± 14.3	77.5 ± 11.3	82.9 ± 9.1	0.080
Biopsied Embryos	10.1 ± 2.98	10.0 ± 2.88	11.9 ± 2.76	10.5 ± 2.78	12.9 ± 2.16
Live Birth	5 (38.5%)	8 (40.0%)	18 (56.3%)	8 (50.0%)	12 (54.5%)	0.705

^aStatistically significant.

Table 2. Sub-group analyses.

		Aneuploidy	Trisomy	Monosomy	Normal Embryo	Fertilization
1 vs.2	p	0.427	0.862	0.387	0.104	0.531
1 vs.3	p	0.001*	0.183	0.090	<0.001*	0.634
1 vs.4	p	0.009	0.299	0.147	0.004*	0.278
1 vs.5	p	<0.001*	0.032	0.001*	<0.001*	0.019
2 vs.3	p	<0.001*	0.065	0.270	0.003*	0.770
2 vs.4	p	0.008	0.193	0.361	0.030	0.726
2 vs.5	p	<0.001*	0.011*	0.005*	<0.001*	0.082
3 vs.4	p	0.726	0.509	0.939	0.606	0.379
3 vs.5	p	0.001*	0.105	0.016	<0.001*	0.015
4 vs.5	p	0.003*	0.133	0.021	0.002*	0.092

Significance levels between groups with regard to rate of aneuploidy, trisomy, monosomy, normal embryo, and fertilization according to post-hoc test with Benferroni correction.

Figure 1. Aneuploidy and normal embryo rates among groups. Ascending numbers from left to right on the X-axis represent the study groups (corresponding to Kruger’s morphological scores). Blue bars show percentage of normal embryos. Lines represent percentage of total aneuploidy (Total AE, Red), Trisomy (Green), and Monosomy (Purple). Values of each line and bars are shown in the bottom of the graph.

Table 3. Correlation analyses (rho test).

	Kruger’s Score		Paternal Age
	rho	p	rho	p
Percentage of Normal Embryo	0.591	<0.001	0.023	0.824
Percentage of Aneuploidy	−0.589	<0.001	−0.131	0.202
Percentage of Trisomy	−0.280	0.004	0.110	0.290
Percentage of Monosomy	−0.360	<0.001	−0.181	0.079
Fertilization	0.244	0.014	0.020	0.848

Maternal age correlation is not included because of no biologic relation with embryos.