Alpha-1 chain of human haptoglobin as viability marker of in vitro fertilized human embryos: information beyond morphology

Figures & data

Table 1. Study characteristics, values are expressed as mean ± SD.

	Study group
Number of patients	86
Number of transferred samples	143
Maternal age	33.8 ± 4.7
Maternal BMI	22.7 ± 2.8
FSH treatment (IU)	1715.9 ± 841.9
Number of follicles	8.0 ± 4.8
No. of metaphase II oocytes	6.7 ± 4.2
Fertilization rate (%)	67.9 ± 27.4

Table 2. Average amount of HptA1 (and standard deviation) based on the clinical outcomes and the results of the related statistical analysis.

	Pregnancy
	No-pregnancy (sd)	Pregnancy (sd)	t	df	p
Worst	65.50 (14.25)	57.51 (10.28)	2.92	64.90	0.004
Best	72.94 (15.01)	54.34 (8.56)	7.25	77.57	2.68e-10
	Live birth
	No live birth (sd)	Live birth (sd)	t	df	p
Worst	64.58 (14.33)	58.72 (10.45)	2.05	49.95	0.045
Best	71.56 (15.56)	54.98 (8.83)	6.12	65.05	5.92e-08

sd: standard deviation; t: value of t-test; df: degrees of freedom; p: significance

Figure 1. Study outline of the main assay steps (A) and the data analysis (B). Spent culture medium samples were stored frozen at –80°C until the haptoglobin alpha-1 assay.

Table 3. Number of pregnancies and no-pregnancies based on ICCS scores and the HptA1 categories. In the parentheses the live birth (or no live birth) data are also shown, where there is no parentheses the values were the same.

	ICCS	HptA1 categories	No-pregnancy	Pregnancy	%
Best scenario	Good	Nonviable	14	1	6.66
		Unclear	12 (13)	9 (8)	42.85 (38.09)
		Viable	5 (6)	7 (6)	58.33 (50.00)
	Fair	Nonviable	15	0	0.00
		Unclear	12	2	14.28
		Viable	2 (4)	7 (5)	77.77 (55.55)
Worst scenario
	Good	Nonviable	14	5	26.31
		Unclear	6 (7)	4 (3)	40.00 (30.00)
		Viable	11 (12)	8 (7)	42.10 (36.84)
	Fair	Nonviable	16	2	11.11
		Unclear	5	1	16.66
		Viable	8 (10)	6 (4)	42.85 (28.57)

Figure 2. Average HptA1 amounts in ICCS groups (1 ‘good’ and 2 ‘fair’) analyzed with Student’s t-test, in case of the worst and the best scenarios.