http://orcid.org/0000-0002-2000-6480
Figures & data
Figure 1. 17β-E2 promoted epithelial-mesenchymal transition and inhibited LINC01541 expression in ESCs. Normal ESCs and LINC01541-overexpressing ESCs were stimulated with 10 nmol/L of 17β-E2 for 48 h. (A) Down-regulated levels of LINC01541 expression in 17β-E2-treated ESCs were measured by RT-qPCR. (B, C) The levels of E-Cadherin, N-Cadherin, and vimentin proteins were detected by western blotting. Cell migration (D) and cell invasion (E) were promoted by 17β-E2 and inhibited by LINC01541 overexpression (Magnification, ×200). ***p < 0.001, vs. the control or 17β-E2 group.
Figure 2. Silencing of LINC01541 promoted the migration and invasion of ESCs. ESCs were transfected with siRNA targeted toward LINC01541 or a recombinant LINC01541 overexpression vector, respectively. (A) LINC01541 expression levels were detected by RT-qPCR. (B, C) The levels of E-Cadherin, N-Cadherin, and vimentin proteins were detected by western blotting. Cell migration (D) and cell invasion (E) were promoted by silencing of LINC01541 and inhibited by LINC01541 overexpression (Magnification, ×200). *p < 0.05, **p < 0.01, ***p < 0.001, vs. vector group; #p < 0.05, ##p < 0.01, ###p < 0.001, vs. si-NC group.
Figure 3. Overexpression of LINC01541 promoted apoptosis in ESCs. ESCs were transfected with siRNA targeted toward LINC01541 or a recombinant LINC01541 overexpression vector, respectively. (A) Cell viability at 12, 24, 36, and 48 h after transfection was measured by the CCK8 assay. (B) Apoptosis of ESCs at 24 h after transfection was detected by flow cytometry. (C, D) The levels of BCL2 and CASP3 proteins were detected by blotting. *p < 0.05, **p < 0.01, ***p < 0.001, vs. vector group; #p < 0.05, ##p < 0.01 vs. si-NC group.
Figure 4. LINC01541 inhibited VEGFA expression and the Wnt/β-catenin signaling pathway. ESCs were stimulated with 10 nmol/L of 17β-E2 for 48 h. (A) The levels of VEGFA mRNA were detected by RT-qPCR. (B) The relative levels of β-catenin and VEGFA were detected by western blotting. **p < 0.01, ***p < 0.001, vs. the control or 17β-E2 group.(C) The levels of VEGFA mRNA in LINC01541 silenced and LINC01541 overexpressing ESCs. (D) The levels of β-catenin and VEGFA proteins in LINC01541 silenced and LINC01541 overexpressing ESCs. *p < 0.05, **p < 0.01, vs. vector group; #p < 0.05, ##p < 0.01, vs. si-NC group.
