Improvement of sperm motility of Oncorhynchus mykiss and Salvelinus fontinalis by L-tryptophan

Figures & data

Table 1. Summary of standard sperm quality parameters (mean ± SD) assessed for S. fontinalis and O. mykiss (n = 6).

	Salvelinus fontinalis		Oncorhynchus mykiss
Parameters	Mean	Range	Mean	Range
Color	White		White
Volume (mL)	4.15 ± 1.26	1–6	8.50 ± 4.24	4–15
pH	7.28 ± 0.15	7.25–7.59	7.26 ± 0.10	7.13–7.35
Spermatocrit (%)	58.20 ± 2.35	55.00–60.00	31.64 ± 4.38	27.63–37.04
Sperm density (×10^9)	8.34 ± 0.34	8.15–8.65	7.26 ± 0.18	7.17–7.38

Table 2. Effect of extenders supplemented with L-tryptophan at different concentrations on mean (±SD) percentages of progressive motility in S. fontinalis sperm after short-term storage.

Storage day (days)	Fresh sperm	Control	0.5 mM	1 mM	2 mM	4 mM
0	89.50 ± 6.14^a,x	90.00 ± 5.00^a,x	90.00 ± 5.00^a,x	87.50 ± 6.45^a,x	85.00 ± 9.13^a,x	83.75 ± 2.50^a,x
1	60.00 ± 0.01^a,y	66.67 ± 5.77^b,y	78.00 ± 9.17^c,y	80.25 ± 0.50^c,x	83.33 ± 2.89^c,x	82.50 ± 2.89^c,x
2	40.00 ± 0.01^a,z	57.20 ± 8.17^b,y	77.00 ± 5.48^c,y	76.00 ± 8.94^c,x	75.00 ± 4.08^c,x	72.50 ± 5.00^c,x
3	0.00 ± 0.00^a,t	15.00 ± 7.07^b,z	59.17 ± 9.17^d,yz	50.80 ± 11.34^d,y	22.50 ± 25.98^c,y	15.00 ± 5.77^b,y
5	0.00 ± 0.00^a,t	13.33 ± 5.77^b,z	58.75 ± 7.50^c,yz	17.00 ± 4.47^b,z	15.00 ± 0.01^b,y	12.50 ± 6.45^b,y
6	0.00 ± 0.00^a,t	0.00 ± 0.00^a,t	54.33 ± 9.43^c,yz	10.00 ± 0.01^b,z	6.67 ± 2.89^ab,yz	4.83 ± 2.50^ab,yz
7	0.00 ± 0.00^a,t	0.00 ± 0.00^a,t	50.83 ± 14.29^b,yz	7.00 ± 0.01^a,z	5.00 ± 0.01^a,yz	3.00 ± 0.01^a,yz
8	0.00 ± 0.00^a,t	0.00 ± 0.00^a,t	44.17 ± 3.76^b,z	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	0.00 ± 0.00^a,z
9	0.00 ± 0.00^a,t	0.00 ± 0.00^a,t	20.00 ± 0.01^b,t	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	0.00 ± 0.00^a,z
10	0.00 ± 0.00^a,t	0.00 ± 0.00^a,t	15.00 ± 0.01^b,t	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	0.00 ± 0.00^a,z
11	0.00 ± 0.00^a,t	0.00 ± 0.00^a,t	8.33 ± 1.53^b,t	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	0.00 ± 0.00^a,z
12	0.00 ± 0.00^a,t	0.00 ± 0.00^a,t	0.00 ± 0.00^a,v	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	0.00 ± 0.00^a,z

^a,b,cDifferent superscripts within the same rows demonstrate significant differences (p < 0.05).

^x,y,z,t,vDifferent superscripts within the same columns demonstrate significant differences (p < 0.05).

Table 3. Effect of extenders supplemented with L-tryptophan at different concentrations on mean (±SD) duration of motility in S. fontinalis sperm after short-term storage.

Storage duration (day)	Fresh sperm	Control	0.5 mM	1 mM	2 mM	4 mM
0	80.50 ± 19.54^a,x	67.33 ± 2.52^a,x	86.00 ± 24.09^a,x	71.50 ± 5.69^a,x	76.25 ± 9.46^a,x	67.50 ± 15.00^a,x
1	40.50 ± 14.06^a,y	61.33 ± 2.31^b,x	81.67 ± 18.93^c,x	64.50 ± 4.20^bc,xy	71.00 ± 9.64^bc,x	64.00 ± 4.90^bc,x
2	20.00 ± 0.01^a,z	58.00 ± 2.74^bc,x	75.33 ± 8.59^d,x	61.00 ± 9.62^c,xy	60.00 ± 0.01^bc,y	51.25 ± 2.50^b,y
3	0.00 ± 0.00^a,t	22.75 ± 7.63^b,y	57.17 ± 11.72^c,y	50.80 ± 8.32^d,y	41.25 ± 10.31^e,y	38.75 ± 2.50^f,z
5	0.00 ± 0.00^a,t	20.00 ± 0.01^b,y	56.67 ± 10.33^d,y	38.00 ± 2.74^c,yz	28.33 ± 2.89^z	27.50 ± 2.89^b,z
6	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	51.67 ± 4.08^d,y	30.00 ± 0.01^c,yz	26.67 ± 2.89^bc,z	25.00 ± 5.77^b,z
7	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	45.83 ± 5.85^b,yz	20.00 ± 0.01^c,z	18.00 ± 0.01^c,t	15.00 ± 0.01^c,t
8	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	40.83 ± 3.76^b,z	0.00 ± 0.00^a,t	0.00 ± 0.00^a,v	0.00 ± 0.00^a,v
9	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	36.67 ± 2.89^b,z	0.00 ± 0.00^a,t	0.00 ± 0.00^a,v	0.00 ± 0.00^a,v
10	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	20.00 ± 0.01^b,t	0.00 ± 0.00^a,t	0.00 ± 0.00^a,v	0.00 ± 0.00^a,v
11	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	12.50 ± 3.54^b,t	0.00 ± 0.00^a,t	0.00 ± 0.00^a,v	0.00 ± 0.00^a,v
12	0.00 ± 0.00^a,t	0.00 ± 0.00^a,z	0.00 ± 0.00^a,v	0.00 ± 0.00^a,t	0.00 ± 0.00^a,v	0.00 ± 0.00^a,v

^a,b,c,d,eDifferent superscripts within the same rows demonstrate significant differences (p < 0.05).

^x,y,z,t,vDifferent superscripts within the same columns demonstrate significant differences (p < 0.05).

Table 4. Effect of extenders supplemented with L-tryptophan at different concentrations on mean (±SD) percentages of progressive motility in O. mykiss sperm after short-term storage.

Storage duration (day)	Fresh sperm	Control	0.5 mM	1 mM	2 mM	4 mM
0	98.00 ± 1.73^a,x	98.00 ± 0.05^a,x	98.00 ± 0.01^a,x	90.00 ± 5.00^ab,x	86.67 ± 5.77^b,x	76.67 ± 5.75^b,x
1	90.00 ± 0.01^a,x	91.67 ± 0.05^a,x	90.00 ± 2.89^a,x	84.67 ± 0.58^ab,x	76.67 ± 5.75^b,xy	66.67 ± 5.70^c,xy
2	0.00 ± 0.00^a,y	82.50 ± 4.47^b,xy	88.00 ± 2.88^b,xy	83.00 ± 1.00^b,x	71.25 ± 2.50^c,xy	53.33 ± 5.71^d,xy
3	0.00 ± 0.00^a,y	72.50 ± 4.46^b,y	78.00 ± 2.85^bc,y	82.00 ± 2.74^c,x	61.25 ± 2.52^d,y	43.33 ± 5.73^e,y
4	0.00 ± 0.00^a,y	37.00 ± 2.50^b,z	38.75 ± 2.80^b,z	37.50 ± 5.00^b,y	26.67 ± 2.89^bc,z	18.33 ± 2.85^c,z
5	0.00 ± 0.00^a,y	26.67 ± 0.01^b,zt	30.00 ± 7.64^b,z	25.00 ± 4.08^b,z	13.33 ± 2.85^c,t	11.67 ± 2.89^c,zt
6	0.00 ± 0.00^a,y	13.33 ± 0.05^b,t	15.26 ± 1.00^c,t	13.00 ± 0.05^bc,t	0.00 ± 0.00^a,v	0.00 ± 0.00^a,t
7	0.00 ± 0.00^a,y	0.00 ± 0.00^a,v	0.00 ± 0.00^a,v	0.00 ± 0.00^a,v	0.00 ± 0.00^a,v	0.00 ± 0.00^a,t

^a,b,c,dDifferent superscripts within the same rows demonstrate significant differences (p < 0.05).

^x,y,z,t,vDifferent superscripts within the same columns demonstrate significant differences (p < 0.05).

Table 5. Effect of extenders supplemented with L-tryptophan at different concentrations on mean (±SD) duration of motility in O. mykiss sperm after short-term storage.

Storage duration (day)	Fresh sperm	Control	0.5 mM	1 mM	2 mM	4 mM
0	47.67 ± 4.51^a,x	113.33 ± 15.28^b,x	108.00 ± 7.21^b,x	76.67 ± 11.55^c,x	73.33 ± 11.54^c,x	70.00 ± 10.00^c,x
1	23.67 ± 0.58^a,y	56.67 ± 7.64^b,y	54.00 ± 3.61^b,y	42.33 ± 2.52^c,y	36.67 ± 5.77^d,y	35.00 ± 5.00^d,y
2	0.00 ± 0.00^a,z	42.50 ± 5.00^b,yz	43.90 ± 5.48^b,z	42.33 ± 0.58^b,y	35.00 ± 4.08^bc,y	28.33 ± 2.89^c,yz
3	0.00 ± 0.00^a,z	40.00 ± 0.50^b,yz	43.75 ± 4.47^b,z	40.75 ± 16.64^b,y	32.67 ± 4.08^bc,y	25.33 ± 2.89^c,yz
4	0.00 ± 0.00^a,z	34.75 ± 10.00^b,z	28.00 ± 2.50^c,t	40.60 ± 4.79^d,y	25.00 ± 2.52^c,yz	18.33 ± 5.03^e,z
5	0.00 ± 0.00^a,z	18.00 ± 1.00^b,t	25.00 ± 5.77^c,t	24.75 ± 0.50^c,z	15.00 ± 0.01^bd,z	12.67 ± 2.62^d,z
6	0.00 ± 0.00^a,z	10.00 ± 0.01^b,t	14.00 ± 0.02^bc,tv	12.00 ± 0.05^c,t	0.00 ± 0.00^a,t	0.00 ± 0.00^a,t
7	0.00 ± 0.00^a,z	0.00 ± 0.00^a,v	0.00 ± 0.00^a,v	0.00 ± 0.00^a,v	0.00 ± 0.00^a,t	0.00 ± 0.00^a,t

^a,b,c,dDifferent superscripts within the same rows demonstrate significant differences (p < 0.05).

^x,y,z,t,vDifferent superscripts within the same columns demonstrate significant differences (p < 0.05).