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APPLICATION NOTE

Individual variation of the percentage of Y-chromosome bearing sperm content in human ejaculates

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Pages 395-398 | Received 15 Jan 2021, Accepted 06 Jun 2021, Published online: 12 Jul 2021

Figures & data

Figure 1. Randomly assayed human ejaculates demonstrate a high level of variability in percentage of Y bearing chromosome

The black dots represent the percent of Y chromosome bearing sperm as determined by a 2−ΔΔCt qPCR quantification method. Overall, the 50 randomly selected participants displayed a high level of variability from the mean. The thick black line represents the mean percentage (45.58 ± 2.87). Additionally, samples displaying the highest deviation from the mean were repeated to ensure accuracy. All samples were performed in triplicate and data was analyzed utilizing Thermo Fisher’s Viia7 Real-Time PCR instrument and Microsoft Excel.
Figure 1. Randomly assayed human ejaculates demonstrate a high level of variability in percentage of Y bearing chromosome

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