The role of preimplantation genetic testing for aneuploidy in a good prognosis IVF population across different age groups

Figures & data

Figure 1. Flow chart of study participant selection

Table 1. Demographics and stimulation characteristics of patients undergoing IVF stimulation cycles (n = 6, 816)

Characteristics	Overall cohort N = 6,816	PGT-A N = 5,077	Non PGT-A/OHSS N = 1,739	P-value
Age (years), mean (SD)	34.9 (4.3)	36.1 (3.9)	31.5 (3.7)	<0.0001
Maternal BMI				0.02
Underweight	202 (3.0)	141 (2.8)	61 (3.5)
Normal	3,389 (49.7)	2,508 (49.4)	881 (50.7)
Overweight	1,199 (17.6)	906 (17.9)	293 (16.9)
Obese	775(11.4)	555 (10.9)	220 (12.7)
Unknown	1,251 (18.4)	967 (19.1)	284 (16.3)
Gravidity, median (IQR)	1 (0–2)	1 (0–2)	0 (0–1)	<0.0001
AMH, median (IQR)	3.6 (2.0–6.3)	3.0 (1.7–5.0)	6.0 (3.8–9.3)	<0.0001
Fertility diagnoses
Male factor	2096 (30.4)	1374 (27.0)	694 (40.0)	<0.0001
Endometriosis	437 (6.4)	322 (6.3)	115 (6.6)	0.69
Polycystic ovarian syndrome	1494 (21.96)	806 (15.9)	688 (39.6)	<0.0001
Diminished ovarian reserve	1191 (17.5)	1135 (22.4)	56 (3.2)	<0.001
Tubal factor	615 (9.0)	405 (8.0)	210 (12.1)	<0.001
Uterine	410 (6.0)	304 (6.0)	106 (6.1)	0.87
Unexplained	967 (14.2)	709 (14.0)	258 (14.8)	0.37
FSH dose, median (IQR)	2,775 (1,975–4,000)	3,150 (2,250–4,275)	2,000 (1,500–2,650)	<0.0001
Total 2 PN, median (IQR)	11 (7–16)	10 (7–14)	15 (11–20)	<0.0001
Oocyte retrieved, median (IQR)	18 (12–25)	16 (11–22)	24 (18–32)	<0.0001
Total embryo frozen, median (IQR)	5 (3–8)	5 (3–7)	7 (5–11)	<0.0001
Agonist suppression	1,125 (16.5)	799 (15.7)	326 (18.8)	0.004
Agonist flare	386 (5.7)	358 (7.1)	28 (1.6)	<0.0001
Antagonist suppression	5,262 (77.2)	3,913 (77.1)	1,349 (77.6)	0.67

SD, standard deviation; IQR, interquartile range; BMI, body mass index.

Percentages may not add to 100 due to rounding.

P values were calculated by independent samples t-test or Wilcoxon-Mann-Whitney test for continuous variables.

P values were calculated by Pearson’s chi-square test for categorical variables

Figure 2. Bar diagram showing the live birth rate (LBR) (A) and miscarriage rate (B) among IVF population without PGT-A (represented in blue) and with PGT-A (represented in orange) across age groups <35 years, 35–37 years, > 37 years. Odds ratios and 95% confidence intervals for the association between live birth and PGT status across all age groups is mentioned on the right box. aOR, adjusted odds ratio; CI, confidence interval

Table 2. Odds ratios and 95% confidence intervals for the association between pregnancy loss, and live birth and PGT-A status in all patients and different age groups

All patients (n = 6,816)	Pregnancy loss		Live birth
Characteristics	OR (95% CI)	aOR (95% CI)	OR (95% CI)	aOR (95% CI)
PGT-A*	1.07 (0.88–1.30)	1.03 (0.82–1.30)	1.07 (0.96–1.19)	1.10 (0.97–1.26)
Age (years)	1.00 (0.98–1.02)	1.00 (0.97–1.02)	1.01 (0.99–1.02)	1.01 (1.00–1.03)
Maternal BMI
Underweight	1.25 (0.78–1.99)	1.28 (0.80–2.05)	1.02 (0.77–1.36)	1.02 (0.77–1.36)
Normal	Ref.	Ref.	Ref.	Ref.
Overweight	1.09 (0.86–1.37)	1.08 (0.86–1.36)	0.91 (0.79–1.03)	0.91 (0.80–1.04)
Obese	1.34 (1.04–1.73)	1.32 (1.02–1.71)	0.77 (0.66–0.90)	0.79 (0.67–0.92)
Gravidity	1.07 (1.02–1.12)	1.07 (1.01–1.12)	0.96 (0.93–0.99)	0.95 (0.92–0.98)
FSH dose, per 450-unit increase	1.01 (0.98–1.03)	1.00 (0.97–1.03)	0.99 (0.98–1.00)	0.99 (0.97–1.00)
Total 2 PN	0.99 (0.98–1.01)	0.98 (0.96–1.01)	1.01 (0.99–1.01)	1.01 (0.99–1.03)
Oocyte retrieved	1.00 (0.99–1.01)	1.01 (0.99–1.02)	1.00 (0.99–1.00)	0.99 (0.98–1.00)
Total embryo frozen	1.00 (0.98–1.02)	1.01 (0.98–1.04)	1.01 (1.00–1.03)	1.02 (0.99–1.03)
<35 years old (n = 2,931)	Pregnancy Loss		Live Birth
Characteristics	OR (95% CI)	aOR (95% CI)	OR (95% CI)	aOR (95% CI)
PGT-A*	1.02 (0.79–1.32)	0.97 (0.72–1.30)	0.97 (0.84–1.12)	1.03 (0.87–1.21)
Maternal BMI
Underweight	1.22 (0.62–2.40)	1.28 (0.65–2.52)	1.15 (0.77–1.72)	1.14 (0.76–1.70)
Normal	Ref.	Ref.	Ref.	Ref.
Overweight	0.91 (0.61–1.34)	0.89 (0.60–1.31)	1.13 (0.92–1.38)	1.13 (0.92–1.39)
Obese	1.63 (1.12–2.38)	1.58 (1.08–2.31)	0.89 (0.70–1.14)	0.92 (0.72–1.17)
Gravidity	1.09 (1.01–1.18)	1.10 (0.78–1.55)	0.96 (0.91–1.01)	0.96 (0.91–1.02)
FSH dose, per 450-unit increase	1.02 (0.98–1.07)	1.02 (0.97–1.07)	0.98 (0.96–1.01)	0.98 (0.96–1.01)
Total 2 PN	1.01 (0.99–1.02)	1.00 (0.97–1.04)	1.00 (0.99–1.01)	1.00 (0.99–1.00)
Oocyte retrieved	1.00 (0.99–1.02)	1.01 (0.99–1.03)	1.00 (0.99–1.01)	1.00 (0.98–1.02)
Total embryo frozen	1.01 (0.98–1.04)	1.00 (0.96–1.04)	1.01 (0.99–1.03)	1.02 (0.99–1.04)
35–37 years old (n = 1,752)	Pregnancy Loss		Live Birth
Characteristics	OR (95% CI)	aOR (95% CI)	OR (95% CI)	aOR (95% CI)
PGT-A*	1.22 (0.77–1.93)	1.11 (0.68–1.82)	1.19 (0.92–1.54)	1.26 (0.96–1.67)
Maternal BMI
Underweight	1.25 (0.52–3.01)	1.28 (0.53–3.08)	1.31 (0.74–2.32)	1.25 (0.71–2.23)
Normal	Ref.	Ref.	Ref.	Ref.
Overweight	1.21 (0.79–1.84)	1.19 (0.78–1.83)	0.77 (0.59–0.99)	0.77 (0.59–0.99)
Obese	1.33 (0.82–2.18)	1.29 (0.79–2.11)	0.75 (0.55–1.02)	0.76 (0.55–1.04)
Gravidity	1.09 (0.99–1.19)	1.08 (0.99–1.19)	0.94 (0.88–0.99)	0.93 (0.88–0.99)
FSH dose, per 450-unit increase	1.03 (0.98–1.08)	1.02 (0.97–1.08)	0.99 (0.96–1.02)	0.99 (0.96–1.02)
Total 2 PN	0.99 (0.96–1.01)	0.98 (0.93–1.03)	1.00 (0.99–1.02)	1.02 (0.99–1.05)
Oocyte retrieved	0.99 (0.98–1.01)	1.00 (0.97–1.04)	1.00 (0.99–1.01)	0.98 (0.97–1.00)
Total embryo frozen	1.00 (0.95–1.04)	1.03 (0.96–1.09)	1.01 (0.99–1.04)	1.02 (0.98–1.06)
37 years old (n = 2,133)	Pregnancy loss		Live birth
Characteristics	OR (95% CI)	aOR (95% CI)	OR (95% CI)	aOR (95% CI)
PGT-A*	1.42 (0.57–3.55)	1.34 (0.52–3.43)	1.23 (0.79–1.91)	1.27 (0.80–2.02)
Maternal BMI
Underweight	1.33 (0.51–3.45)	1.30 (0.50–3.38)	0.65 (0.36–1.16)	0.67 (0.37–1.20)
Normal	Ref.	Ref.	Ref.	Ref.
Overweight	1.22 (0.82–1.80)	1.22 (0.82–1.82)	0.78 (0.62–0.98)	0.79 (0.63–0.99)
Obese	1.03 (0.63–1.68)	1.04 (0.63–1.71)	0.64 (0.49–0.85)	0.67 (0.51–0.89)
Gravidity	1.03 (0.94–1.12)	1.03 (0.94–1.13)	0.96 (0.91–1.01)	0.96 (0.92–1.02)
FSH dose, per 450-unit increase	0.98 (0.94–1.03)	0.97 (0.92–1.02)	0.98 (0.95–1.01)	0.99 (0.96–1.02)
Total 2 PN	0.97 (0.94–0.99)	0.94 (0.89–0.99)	1.02 (1.00–1.03)	1.03 (0.99–1.06)
Oocyte retrieved	0.99 (0.97–1.01)	1.02 (0.99–1.05)	1.00 (0.99–1.01)	0.98 (0.97–1.00)
Total embryo frozen	0.96 (0.91–1.01)	1.00 (0.94–1.07)	1.03 (1.01–1.06)	1.02 (0.98–1.06)

OR, unadjusted odds ratio; aOR, adjusted odds ratio; CI, confidence interval; BMI, body mass index.

*Reference group is non PGT-A/OHSS.

Bolded values are for confidence intervals that do not contain 1.00.