Figures & data
Figure 1. Scheme of ultrasensitive detection of proteins by ELISA coupled with thio-NAD cycling. Our ultrasensitive ELISA requires only the addition of a thio-NAD cycling solution, which includes androsterone derivatives, and 3α-hydroxysteroid dehydrogenase and its coenzymes, to the usual ELISA without any use of special instruments. Absorption (400 nm) of thio-NADH is measured with a commercially available microplate reader.
Figure 2. Scheme of ultrasensitive detection of sugars by FCS.The concentrations of H2O2 correspond to those of the proteins labeled with tyramide-tetramethyl rhodamine (TMR). H2O2 is produced by the reaction between glucose and glucose oxidase, and thus we can determine the concentrations of H2O2 and deduce the concentrations of glucose by FCS.
