1,901
Views
17
CrossRef citations to date
0
Altmetric
Article Addendum

Oral delivery of dsRNA by microbes: Beyond pest control

&
Article: e1236163 | Received 28 Jul 2016, Accepted 07 Sep 2016, Published online: 04 Nov 2016

Figures & data

Figure 1. Schematic summarizing the protocol for production and oral delivery of genetically modified yeast expressing dsRNA to insect targets. Inverted repeats of target sequence are cloned into expression vector p406TEF1 and transformed into S. cerevisae. Transformants are selected on minimal media without Uracil. Previously expanded yeast culture is then pelleted and could be use for further applications. For more details, see ref. 2.

Figure 1. Schematic summarizing the protocol for production and oral delivery of genetically modified yeast expressing dsRNA to insect targets. Inverted repeats of target sequence are cloned into expression vector p406TEF1 and transformed into S. cerevisae. Transformants are selected on minimal media without Uracil. Previously expanded yeast culture is then pelleted and could be use for further applications. For more details, see ref. 2.