A novel host factor for human respiratory syncytial virus

Figures & data

Figure 1. Filopodia-driven RSV cell-to-cell spread. A549 cells were infected with RSV wild type at a multiplicity of infection of 1 for 24 hr. Cells were fixed, permeabilized, and immunostained for RSV F (green) by incubating with mouse monoclonal antibody specific for the RSV F protein (abcam, ab43812) followed by incubating with the secondary antibody anti-mouse AlexaFluor₄₈₈ (Life Technologies). The cells were further stained with rhodamine phalloidin (Cytoskeleton Inc.) to detect F-actin (red). Images were collected on a Leica TCS SP8 STED 3X system (Leica Microsystems) as described in.⁷ This image illustrates that the filopodia (indicated by arrows) of the RSV-infected cell (labeled A) appear to convey RSV particles (indicated by arrow heads) to a neighboring cell (labeled B).

Mehedi M, McCarty T, Martin SE, Le Nouen C, Buehler E, Chen YC, Smelkinson M, Ganesan S, Fischer ER, Brock LG, et al. Actin-Related Protein 2 (ARP2) and Virus-induced Filopodia facilitate human respiratory syncytial virus spread. PLoS Pathog 2016; 12(12):e1006062; PMID:27926942; https://doi.org/10.1371/journal.ppat.1006062

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