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Short Communication

Exopolysaccharide-mediated surface penetration as new virulence trait in Enterococcus faecalis

& ORCID Icon
Pages 144-147 | Received 01 Aug 2019, Accepted 15 Aug 2019, Published online: 26 Aug 2019

Figures & data

Figure 1. Enterococcal surface penetration.

(a–c). The capacity of E. faecalis MMH594 to penetrate was evidenced as a colony-print inside the agar after removing the non-penetrating cells (outside) through several washes with water. (a) Images of a 6-day-old colony (outside) and its penetrating cells (inside) grown on semisolid medium at 37°C. (b) Scanning electron microscopy of aggregated and matrix-covered (purple arrow head) enterococcal cells. (c) Top view of a colony and its invading community (side view) demonstrating the spatial localization of discrete E. faecalis aggregated communities differentially labeled with either red (m-Cherry) or green (GFP) fluorescent proteins. The top white line indicates the beginning of the agar. Scale bars: 1 mm (a and c); 2 μm (b) and 100 μm (d). Methodological details are further described in reference [Citation8].

Figure 1. Enterococcal surface penetration.(a–c). The capacity of E. faecalis MMH594 to penetrate was evidenced as a colony-print inside the agar after removing the non-penetrating cells (outside) through several washes with water. (a) Images of a 6-day-old colony (outside) and its penetrating cells (inside) grown on semisolid medium at 37°C. (b) Scanning electron microscopy of aggregated and matrix-covered (purple arrow head) enterococcal cells. (c) Top view of a colony and its invading community (side view) demonstrating the spatial localization of discrete E. faecalis aggregated communities differentially labeled with either red (m-Cherry) or green (GFP) fluorescent proteins. The top white line indicates the beginning of the agar. Scale bars: 1 mm (a and c); 2 μm (b) and 100 μm (d). Methodological details are further described in reference [Citation8].