DnaJ molecules as potential effectors in Meloidogyne arenaria. An unexplored group of proteins in plant parasitic nematodes

Figures & data

Table 1. Nematode species used in this study and the number of predicted DnaJ by proteome.

Species	Lifestyle	Protein number	BioProject^a	Query^b	Number of DNAj	Number of secreted DNAj^c
Caenorhabditis brenneri	Free-living	30,017	PRJNA20035	G0N2B2_CAEBE	36	4
Caenorhabditis briggsae	Free-living	23,913	PRJNA10731	A8XMW5_CAEBR	36	5
Caenorhabditis elegans	Free-living	28,196	PRJNA13758	Q9XWE1_CAEEL	34	3
Caenorhabditis japónica	Free-living	35,013	PRJNA12591	A0A2H2HXM1_CAEJA	33	4
Caenorhabditis remanei	Free-living	31,235	PRJNA53967	A0A260YRA3_CAERE	26	3
Pristionchus pacificus	Free-living	25,204	PRJNA12644	A0A2A6BSD8_PRIPA	24	3
Ascaris suum	Animal parasite	46,912	PRJNA62057	F1KVF4_ASCSU	72	4
Brugia malayi	Human parasite	14,293	PRJNA10729	A0A0K0J867_BRUMA	36	3
Loa loa	Human parasite	15,319	PRJNA37757	A0A1I7VI53_LOALO	31	3
Wuchereria bancrofti	Human parasite	13,053	PRJEB536	A0A1I8EK19_WUCBA	25	1
Trichinella spiralis	Human parasite	16,067	PRJNA257433	A0A0V1B3E5_TRISP	20	3
Bursaphelenchus xylophilus	Plant parasite	17,652	PRJEA64437	A0A0K0F543_STRVS	28	2
Globodera pallida	Plant parasite	16,312	PRJEB123	A0A183CPU2_GLOPA	36	1
Meloidogyne hapla	Plant parasite	14,378	PRJNA29083	A0A1I8AZ83_MELHA	32	4
Meloidogyne incognita	Plant parasite	21,673	PRJNA340324	A0A2L0VY59_MELAR	47	8
Meloidogyne arenaria	Plant parasite	98,472	PRJEB8714	A0A2L0VY59_MELAR	141	32

^aBioProject ID where the proteome was extracted

^bUniprot ID of the protein used as a query in the PSI BLAST analysis

^cThe number of secreted proteins was calculated according to the predictions of SignalP

Figure 1. Maximum likelihood tree reconstructed with DNAj proteins found in Meloidogyne arenaria. The accession numbers highlighted in red correspond to the members of the DNAj type I proteins, the blue labels correspond to DNAj type II proteins, black labels to DNAj type III proteins and green labels to DNAj type IV proteins, in concordance with Rug and Maier [23]. Blue light labels correspond to DnaJ proteins with J domains and DnaJ C-terminal domains and yellow labels correspond to the putative secreted proteins. The tree was supported by FastTree support value.

Figure 2. Alignment between M.Arenaria_Scaff164g004450 and MG599854. Domain annotations are highlighted behind the alignment.

Figure 3. MG599854 gene expression on tomato-root samples infected with Meloidogyne arenaria at 0, 3, 5, 7, 9, 14, and 21 dpi (days post infection). MG599854 expression is visible from 5 dpi and progressively increases until 25 dpi. GADH gene was used as internal control.

Figure 4. Ectopic expression of MG599854 by agroinfiltration technique in Nicotiana benthamiana leaves assessed at 3 days post inoculation (dpi). INF1 and GPF genes are positive and negative controls, respectively. Cell death is visible in MG599854 and INF1 gene expression (marked with a circle on the leaf).

Rug M, Maier AG. The heat shock protein 40 family of the malaria parasite Plasmodium falciparum. IUBMB Life. 2011;63:1081–1086.

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