https://orcid.org/0000-0002-0812-1566
Figures & data
Figure 1. AtAGO4 does not localize in the Cajal body when transiently expressed in N. benthamiana. A. Alignment of AGO4 proteins from Arabidopsis and N. benthamiana. The alignment was performed by using ClustalW. The identities between AtAGO4 and NbAGO4-1, AtAGO4 and NbAGO4-2, and NbAGO4-1 and NbAGO4-2 are 73.5%, 72.5%, and 86.8%, respectively. B. Subcellular localization of GFP-AtAGO4 and GFP-NbAGO4-1 in N. benthamiana leaves. Fibrillarin-RFP is used as a nucleolus and Cajal body marker. GFP-AtAGO4 or GFP-NbAGO4-1 were co-expressed with Fibrillarin-RFP in N. benthamiana leaf epidermal cells by A. tumefaciens-mediated transient gene expression. Confocal images were taken two days after infiltration. Arrowheads indicate the position of the Cajal body. Bar, 10 μm. This experiment was repeated three times with similar results. C. Quantification of Cajal body localization of GFP-NbAGO4-1 or GFP-AtAGO4 in N. benthamiana leaves.
