https://orcid.org/0000-0002-2766-3889
Figures & data
Figure 1. KN1 mRNAs are detected at low levels in L1 (epidermal) cells of maize meristems. (a) Single-cell RNA sequencing [Citation42] indicates that KN1 transcripts are abundant in meristem (clusters 9, 10, and 11), vasculature (clusters 4, 5, and 12), and ground tissue (clusters 1 and 8), but also present at low levels in meristem L1 cells (cluster 6, asterisks). (b) Over-exposure of a KN1 mRNA in situ hybridization shows a weak signal in the L1 (pink) and a strong signal in the inner meristem layers (dark blue) in a maize ear spikelet pair meristem. (c-g) Rice OSH1 mRNA is absent from the L1 layer of the vegetative shoot apical meristem (SAM) (c) but observed in some L1 cells in the inflorescence meristem (im) (d), and is throughout the L1 in the spikelet meristem (sm) (e) and floret meristem (fm) (f), then is again restricted to the inner layers in the later stage fm (g). P0 and P1, plastochron 0 and 1; rg, rudimentary glume; sl, sterile lemma; ca, carpel. (h) mRNA in situ hybridization showing STM mRNA in the entire vegetative shoot meristem including L1 layer in Arabidopsis. The data used for panel A is from [Citation42]. Panel C, D, E, F-G, and H used images from [Citation48,Citation54–56] and [Citation8] with permission, respectively. Scale bars = 50 µm.
![Figure 1. KN1 mRNAs are detected at low levels in L1 (epidermal) cells of maize meristems. (a) Single-cell RNA sequencing [Citation42] indicates that KN1 transcripts are abundant in meristem (clusters 9, 10, and 11), vasculature (clusters 4, 5, and 12), and ground tissue (clusters 1 and 8), but also present at low levels in meristem L1 cells (cluster 6, asterisks). (b) Over-exposure of a KN1 mRNA in situ hybridization shows a weak signal in the L1 (pink) and a strong signal in the inner meristem layers (dark blue) in a maize ear spikelet pair meristem. (c-g) Rice OSH1 mRNA is absent from the L1 layer of the vegetative shoot apical meristem (SAM) (c) but observed in some L1 cells in the inflorescence meristem (im) (d), and is throughout the L1 in the spikelet meristem (sm) (e) and floret meristem (fm) (f), then is again restricted to the inner layers in the later stage fm (g). P0 and P1, plastochron 0 and 1; rg, rudimentary glume; sl, sterile lemma; ca, carpel. (h) mRNA in situ hybridization showing STM mRNA in the entire vegetative shoot meristem including L1 layer in Arabidopsis. The data used for panel A is from [Citation42]. Panel C, D, E, F-G, and H used images from [Citation48,Citation54–56] and [Citation8] with permission, respectively. Scale bars = 50 µm.](/cms/asset/6bab296f-4b8a-4ff3-ad8f-ddcf75434c9a/kcib_a_2095125_f0001_oc.jpg)