Dual localization of the carboxy-terminal tail of GLR3.3 in sieve element–companion cell complex

Figures & data

Figure 1. Functional and subcellular characterization of GLR3.3CT. (a) Representative traces of the SWPs in both L8 (black trace) and L13 (blue trace) from WT, glr3.3a mutants and two independent lines of transgenic plants expressing GLR3.3CT-mVENUS fusions (GLR3.3CT-1 and GLR3.3CT-2). (b) Amplitude (upper panel) and duration (lower panel) quantification results of the L13 SWPs in different materials. Yellow circles represent individual recordings. n = 11–16. Data shown are mean ± SD. The different letters indicate significant differences after one-way ANOVA. (c-d) Subcellular localization of GLR3.3CT-mVENUS fusions. In (c), yellow is the signal from GLR3.3CT-mVENUS fusion protein. The junction between two sieve tubes was shown in the zoomed red box with dotted line. In (d), DAPI staining (blue) was further used to visualize the nuclei. Asterisks mark the positions of sieve plates. Bar = 2 μm in the enlarged image. Bar = 10 μm in all the other cases. (e) Amino acid sequence of GLR3.3 C-tail with a putative NLS highlighted by red. The NLS was predicted using cNLS Mapper [27].

Kosugi S, Hasebe M, Tomita M, et al. Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs. Proc Natl Acad Sci U S A. 2009;106:10171–10176.

 PubMed Web of Science ®Google Scholar