Immunohistochemical Expression of Adult Stem Markers in Salivary Mucoepidermoid Carcinoma with Relevance to Molecular Profiling: Any Prognostic Implications and Oncogenic Mechanisms Eked?

Figures & data

Table 1. Clinicopathological profile of the 40 parotid cases.

Case	Sex/age	Diagnosis	cTNM	CD133	CD44	OCT4	MENA	LN	rPNI	Clinical prognosis
1	44/M	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
2	49/F	IG-MEC	cT2N1M0	-	-	-	++++	No	No	Good
3	52/M	IG-MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
4	57/F	IG-MEC	cT2N0M0	-	-	-	++++	Yes	Yes	Favorable
5	42/M	HG-MEC	cT1N2M1	-	-	-	++++	Yes	Yes	Poor
6	40/F	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
7	42/M	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
8	34/M	IG-MEC	cT2N1M0	-	-	-	++++	No	No	Good
9	40/F	IG-MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
10	43/M	IG-MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
11	43/M	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
12	56/F	IG-MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
13	45/M	Low-grade MEC	cT2N0M0	-	-	-	++++	Yes	Yes	Favorable
14	54/F	Low-grade MEC	cT2N0M0	-	-	-	++++	Yes	Yes	Favorable
15	62/F	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
16	57/F	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Poor
17	42/M	IG-MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
18	39/F	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
19	12/M	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
20	34/M	IG-MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
21	19/M	HG-MEC	cT2N2M1	-	-	-	++++	Yes	Yes	Favorable
22	34/F	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
23	41/F	IG-MEC	cT2N0M1	-	-	-	++++	No	No	Poor
24	47/M	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
25	28/F	IG-MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
26	36/M	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
27	50/F	Low-grade MEC	cT2N1M0	-	-	-	++++	No	No	Poor
28	39/M	Low-grade MEC	cT2N0M0	-	-	-	++++	Yes	Yes	Favorable
29	46/F	Low-grade MEC	cT2N0M0	-	-	-	++++	Yes	Yes	Favorable
30	31/M	IG-MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
31	58/F	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
32	42/M	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
33	40/F	IG-MEC	cT3N1M0	-	-	-	++++	No	No	Favorable
34	42/M	HG-MEC	cT3N2M1	-	-	-	++++	No	No	Poor
35	34/M	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
36	40/F	IG-MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
37	43/M	Low-grade MEC	cT2N0M0	-	-	-	++++	Yes	Yes	Favorable
38	43/M	IG-MEC	cT2N0M0	-	-	-	++++	Yes	Yes	Favorable
39	56/F	Low-grade MEC	cT2N0M0	-	-	-	++++	No	No	Favorable
40	45/M	IG-MEC	cT2N0M0	-	-	-	++++	No	No	Favorable

Abbreviations: F: Female; IG-MEC: Intermediate grade MEC; LN: Lymph node involvement; M: Male; rPNI: Radiographic perineural invasion.

Table 2. Histologic profile of the 40 parotid cases and their grading according to the AFIP system.

Case	Cysts	PNI	Necrosis	Mitoses/10 HPF	Anaplasia	LVI	Invasive front	Bony Invasion	AFIP grade
1	15	Yes	Yes	4	Yes	No	Yes	Yes	Low
2	40	No	No	3	No	No	No	No	IG
3	25	Yes	Yes	3	No	No	Yes	No	IG
4	20	Yes	Yes	3	No	Yes	Yes	No	IG
5	35	Yes	Yes	3	No	Yes	Yes	No	High
6	20	Yes	No	3	Yes	No	Yes	No	Low
7	40	Yes	No	2	No	Yes	No	No	Low
8	45	No	No	1	No	No	No	No	IG
9	40	No	No	3	No	No	No	No	IG
10	20	Yes	No	3	Yes	No	Yes	No	IG
11	30	No	Yes	1	No	No	No	No	Low
12	20	No	No	2	No	No	No	No	IG
13	25	Yes	Yes	3	No	Yes	Yes	No	Low
14	35	No	No	2	No	No	No	No	Low
15	30	No	No	1	No	No	No	No	Low
16	20	No	No	2	No	No	No	No	Low
17	45	Yes	Yes	3	No	No	Yes	Yes	IG
18	40	Yes	No	2	No	Yes	No	No	Low
19	30	No	No	1	No	No	No	No	Low
20	20	Yes	Yes	3	No	Yes	Yes	No	IG
21	15	Yes	Yes	4	No	No	Yes	Yes	High
22	35	No	No	2	No	No	No	No	Low
23	40	Yes	No	2	No	Yes	No	No	IG
24	40	No	No	3	No	No	No	No	Low
25	25	Yes	Yes	3	No	No	Yes	No	IG
26	20	No	No	2	No	No	No	No	Low
27	30	No	No	1	No	No	No	No	Low
28	15	Yes	Yes	4	No	No	Yes	Yes	Low
29	30	Yes	Yes	3	No	Yes	Yes	No	Low
30	40	No	No	3	No	No	No	No	IG
31	20	Yes	Yes	4	Yes	No	Yes	Yes	Low
32	20	Yes	Yes	3	No	Yes	Yes	No	Low
33	35	No	No	2	No	No	No	No	IG
34	45	Yes	Yes	3	No	No	Yes	Yes	High
35	30	No	Yes	1	No	No	No	No	Low
36	25	Yes	Yes	3	No	Yes	Yes	No	IG
37	20	Yes	No	3	Yes	No	Yes	No	Low
38	20	Yes	No	3	No	No	No	No	IG
39	40	Yes	No	3	Yes	No	Yes	No	Low
40	35	No	No	2	No	No	No	No	IG

Abbreviations: IG: Intermediate grade; LVI: Lymphovascular invasion; PNI: perineural invasion.

Figure 1. Micrograph showing (a) Immunoreactive deposits appeared as reddish-brown reactions where the target antigen was located (MENA) in conventional low-grade MEC (40×); (b) Negative immunostaining for CD133 in low-grade oncocytic MEC (40×); (c) Negative immunostaining for CD44 in low-grade oncocytic MEC (40×). (d) Negative immunostaining for OCT4 in high-grade solid MEC (10×).

Figure 2. Photomicrograph showing retained signals for POU5F1 FISH probe.