Comparison of the dissipation behaviour of three neonicotinoid insecticides in tea

Abstract

The dissipation behaviour of three neonicotinoids – thiamethoxam, imidacloprid and acetamiprid – was compared in tea shoots, in Chinese green and black tea, and after tea infusion in hot water. The simple and rapid analytical procedures for the quantification of these three residues in these matrices were developed using HPLC with ultraviolet (UV) detection. Degradation rates in tea shoots of neonicotinoids applied in either recommended or double dosages followed first-order kinetics, with half-lives of 1.62 or 1.58 days for thiamethoxam, of 2.45 or 2.67 days for imidacloprid, and of 3.24 or 3.85 days for acetamiprid, respectively. Through harvest and processing the residue retentions for thiamethoxam, imidacloprid and acetamiprid were 85.0%, 84.1% and 70.6% of the initial dosages in green tea, and 77.1%, 52.4% and 57.4% in black tea. These three residues all showed high transfer rates through green or black tea brewing of 80.5% or 81.6% for thiamethoxam, of 63.1% or 62.2% for imidacloprid, and of 78.3% or 80.6% for acetamiprid. Waiting periods between the last application and harvest of at least 12, 17 and 20 days were suggested for thiamethoxam, imidacloprid and acetamiprid, respectively, after application at their recommend dosages to ensure levels below a maximum residue limit (MRL) of 0.05 mg kg⁻¹.

Keywords:

• green tea
• black tea
• tea infusion
• neonicotinoids
• pesticide residue
• harvest waiting period

Acknowledgments

The authors thank Mr. Shao-de Hu, Tea Experimental Farm and Factory of Anhui Agricultural University, for tea processing measurement. This work was supported by the National Natural Science Foundation of China (Grant Number 31270728) and The National Spark Program (Grant Number 2012GA710001).