Abstract
Rosa roxburghii (R. roxburghii) is a unique, edible, medicinal fruit rich in vitamin C found in Southwest China. Triadimefon (TDF) is a triazole fungicide that is widely used to control powdery mildew in R. roxburghii. To assess the safety of TDF in R. roxburghii, an LC-MS/MS method was developed for the simultaneous quantification of TDF and its major metabolite, triadimenol (TDN) in R. roxburghii. Both TDF and TDN showed high correlation coefficients (>0.999) for the solvent- and matrix-matched calibrations. The recovery rates of TDF and TDN in R. roxburghii ranged from 90.18% to 100.42%, with a relative standard deviation (RSD) of 1.25%–9.22%. The limit of quantification (LOQ) was 0.01 mg·kg−1. The half-life of TDF in R. roxburghii was between 2.74 and 3.07 days, with terminal residues ranging from < LOQ to 1.84 mg·kg−1. Recommended maximum residue limits (MRLs) and safe pre-harvest intervals (PHIs) for TDF in R. roxburghii were 0.5 mg·kg−1 and 21 days, respectively. This study provides essential data for TDF’s safe and judicious use in R. roxburghii production.
Acknowledgments
Authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (No. 32160656), Guizhou Provincial Innovation Talent Team (No. CXTD [2023]015), the Science-Technology Support Program of Guizhou Province (No. [2021]YB243), and the Hundred Level Talent Foundation of Guizhou Province (No. GCC [2022]023-1).
Data availability statement
The authors confirm that all data generated or analyzed during this study are available within the article.