Abstract
Recently, a number of microcontaminants were found in aquatic environment and it raises the concerns about their effects to ecosystem. This study investigated if low levels of antibiotics can trigger the biofilm formation and result in the enhanced antibiotic gene transfer. For this purpose, the biofilm formation of enteric bacteria (Escherichia coli), environmental micro-organism (Pseudomonas aeruginosa), and their mixture by ppb level of antibiotics were investigated. In addition, the effects on the conjugation of E. coli with P. aeruginosa in the biofilm structure were also evaluated using biofilm colony-forming unit assay in 96-well plates and ELISA. Interestingly, at 100–1000 ppb, the mixed culture was able to reach its highest biofilm biomass and also form the highest number of transconjugants, which is greater than negative controls. This experiment shows that ppb levels of tetracycline and cephradine can alter the transfer rate of the pB10 plasmid among the biofilm biomass at rates 2–5 times faster than without antibiotics, which indicated the facilitated Antibiotic Resistance Gene (ARG) spread by the presence of low-antibiotic residues in the environment.
Acknowledgment
This study was supported by Korea Ministry of Environment as “Global Top Project” [Project no: GT-11-B-01-005-1]. Sincere thanks to Yeji Lee from the Laboratory of Molecular Immunology and Microbial Pathogens for her technical assistance in the biofilm experiment and for providing P. aeruginosa P680 PAK exoTGm.
Notes
Presented at the 5th IWA-ASPIRE Conference, 8–12 September 2013, Daejeon, Korea