Fecal microbiota transplantation significantly improved respiratory failure of amyotrophic lateral sclerosis

Figures & data

Table 1. Scores of the ALS functional rating scale before and after FMT.

Item		Case 1			Case 2
		Before FMT	After the first FMT	After the second FMT	Before FMT	After the first FMT	After the second FMT
Bulbar	1. Speech	4	4	4	4	4	4
	2. Salivation	4	4	4	2	2	4
	3. Swallowing	1	4	4	1	1	1
Fine motor	4. Handwriting	1	1	1	1	1	3
	5a. Cutting food and handling utensils (patients without gastrostomy)	0	0	0	-	0	0
	5b. Cutting food and handling utensils (alternate scale for patients with gastrostomy)	-	-	-	1	-	-
Gross motor	6. Dressing and hygiene	0	0	0	0	0	0
	7. Turing in bed and adjusting bed clothes	0	0	0	1	1	2
	8. Walking	1	1	2	1	1	2
	9. Climbing stairs	0	0	0	0	1	1
Respiratory	10. Dyspnea	0	0	0	0	0	1
	11. Orthopnea	4	4	4	4	4	4
	12. Respiratory insufficiency	0	0	1	0	1	1
Total		15	18	20	15	16	23

Figure 1. Microbiome analysis of Case 1 ALS patient and donor intestinal bacteria by metagenomics sequencing.

The patient’s stool microbiomes were longitudinally sampled at indicated time points before and after FMT, along with samples from the donor.

(a) Alpha diversity, quantified by the inverse Shannon’s index, was evaluated for Case 1 patient and donor samples. (b) The PCoA ordination of weighted UniFrac distances between Case 1 patient and donor. Microbiome samples are depicted in space with more similar samples located closer together. (c) The relative abundance of top 20 dominant taxa at genus level in each sample.

Figure 2. Microbiome analysis of Case 2 ALS patient and donor intestinal bacteria by metagenomics sequencing.

The patient’s stool microbiomes were longitudinally sampled at indicated time points before and after FMT, along with samples from the donor.

(a) Alpha diversity, quantified by the inverse Shannon’s index, was evaluated for Case 2 patient and donor samples. (b) The PCoA ordination of weighted UniFrac distances between Case 2 patient and donor. Microbiome samples are depicted in space with more similar samples located closer together. (c) The relative abundance of top 20 dominant taxa at genus level in each sample.

Data availability statement

The original metagenomic sequencing and metabolomic data in this study have been deposited in the National Center for Biotechnology Information and will be accessible with accession numbers PRJNA1025870 and MTBLS8716 upon publication.