https://orcid.org/0000-0001-8904-9502
Figures & data
Table 1. Summary of eIF4E partner proteins.
Figure 1. A, Single section of confocal imaging of U2OS cells stained for eIF4E (sc-271480 anti-eIF4E antibody, red) and DAPI (blue, nuclear dye) showing nuclear and cytoplasmic localization. Left panel shows the overlap between DAPI and eIF4E staining. The right panel shows eIF4E signal alone. B, Confocal imaging of eIF4E in the nucleus of HeLa cells with the antibody 10C6 from [Citation101]. Reprint with permission from Dostie J, Lejbkowicz F, Sonenberg N. Nuclear eukaryotic initiation factor 4E (eIF4E) colocalizes with splicing factors in speckles [Citation99]. White bar = 10μm.
![Figure 1. A, Single section of confocal imaging of U2OS cells stained for eIF4E (sc-271480 anti-eIF4E antibody, red) and DAPI (blue, nuclear dye) showing nuclear and cytoplasmic localization. Left panel shows the overlap between DAPI and eIF4E staining. The right panel shows eIF4E signal alone. B, Confocal imaging of eIF4E in the nucleus of HeLa cells with the antibody 10C6 from [Citation101]. Reprint with permission from Dostie J, Lejbkowicz F, Sonenberg N. Nuclear eukaryotic initiation factor 4E (eIF4E) colocalizes with splicing factors in speckles [Citation99]. White bar = 10μm.](/cms/asset/4d014c37-407b-4ed0-b0f8-c6e834f496ed/kncl_a_2360196_f0001_oc.jpg)
Figure 3. eIF4E can directly influence RNA fate or indirectly through its capacity to terraform the RNA processing landscape. On the left, interactions between eIF4E target mRNAs and the noted machineries are shown. On the right, numbers of mRNAs in a given process were identified in nuclear, endogenous eIF4E RIP-Seq [Citation54] GSE63265_LY1_4EIP_allreps_counts.Txt.gz and/or RNA-seq splicing data segregated on high and normal-eIF4E AML specimens [Citation100] (https://leucegene.ca/). These strongly suggest that eIF4E can influence a broad array of factors responsible for RNA processing thereby terraforming the RNA processing landscape. Functional categories were assigned using Metascape (metascape.Org).
![Figure 3. eIF4E can directly influence RNA fate or indirectly through its capacity to terraform the RNA processing landscape. On the left, interactions between eIF4E target mRNAs and the noted machineries are shown. On the right, numbers of mRNAs in a given process were identified in nuclear, endogenous eIF4E RIP-Seq [Citation54] GSE63265_LY1_4EIP_allreps_counts.Txt.gz and/or RNA-seq splicing data segregated on high and normal-eIF4E AML specimens [Citation100] (https://leucegene.ca/). These strongly suggest that eIF4E can influence a broad array of factors responsible for RNA processing thereby terraforming the RNA processing landscape. Functional categories were assigned using Metascape (metascape.Org).](/cms/asset/be0a4f97-1604-4b27-bd90-a94400d7932b/kncl_a_2360196_f0003_oc.jpg)
Table 2. Summary of RNA processing factors that are regulated by eIF4E.
Figure 2. The human eIF4E structure binding the m7G cap (in red) and known interactions: eIF4E structure and relative position of regions utilized by known partner proteins. The red arrow indicates the cap-binding site with the associated tryptophans in light blue, the purple arrow indicates the positively charge patch, where Importin 8, VPg and EG5 bind with the 2 lysines and the arginine shown. The dorsal surface is also shown and as is the surface used by the NC (non- canonical domain) of eIF4G and 4EBPs with gold yellow arrows (Adapted from [Citation52], PDB3AM7).
![Figure 2. The human eIF4E structure binding the m7G cap (in red) and known interactions: eIF4E structure and relative position of regions utilized by known partner proteins. The red arrow indicates the cap-binding site with the associated tryptophans in light blue, the purple arrow indicates the positively charge patch, where Importin 8, VPg and EG5 bind with the 2 lysines and the arginine shown. The dorsal surface is also shown and as is the surface used by the NC (non- canonical domain) of eIF4G and 4EBPs with gold yellow arrows (Adapted from [Citation52], PDB3AM7).](/cms/asset/bfd02bb6-7071-4880-ad25-307080f07493/kncl_a_2360196_f0002_oc.jpg)
Data availability statement
Data sharing is not applicable to this article as no new data were created or analyzed in this study.