Effects of Ecballium elaterium on brain in a rat model of sepsis-associated encephalopathy

Figures & data

Table 1. Biochemical analysis of brain tissue in different groups.

	IL-1β ± sd	IL-6±sd	TNF-α ± sd	TAC ± sd	TOS ± sd
	pg/L	pg/mL	ng/L	mmol/L	μmol/L
CG	392.9 ± 12.5	31.7 ± 2.2	51.9 ± 2.4	0.1 ± 0.06	116.4 ± 11.6
SG	403.5 ± 35.0	29.9 ± 1.2	59.5 ± 8.4	0.2 ± 0.03	99.3 ± 16.9
TG	374.7 ± 37.5	36.8 ± 9.5	50.2 ± 6.2	0.2 ± 0.09	82.3 ± 7.8
p^a	0.796	0.06	0.003	0.004	0.034
p^b	0.08	0.02	0.007	0.21	0.01

CG: control group; SG: sepsis group; TG: treatment group; p^a: comparison of CG and SG; p^b; comparison of SG and TG.

Table 2. Biochemical analysis of brain tissue in different groups. Median (min-max).

	IL-1β pg/L	IL-6 pg/mL	TNF-α ng/L	TAC mmol/L	TOS μmol/L
CG	396,9 (371,6–403,7)	31,2 (29,6–34,6)	52,8 (47,8–54,6)	0,12 (0,02–0,23)	113,6 (101,5–134,9)
SG	394,5 (349,6–498,4)	29,8 (27,8–31,9)	55,7 (52,8–75,0)	0,22 (0,14–0,25)	95,2 (76,2–127,6)
TG	377,7 (327,3–431,2)	34,4 (27,6–54,6)	49,3 (38,3–61,1)	0,28 (0,13–0,43)	83,7 (72,03–95,7)
p^a	0.796	0.06	0.003	0.004	0.034
p^b	0.08	0.02	0.007	0.21	0.01

CG: control group; SG: sepsis group; TG: treatment group.

Table 3. Brain histopathology results.

	CG	SG	TG	p^a	p^b
Neuronal damage ± sd	0.00	2.3 ± 0.4	0.00	0.002	0.00
Edema and inflammatory cell infiltration ± sd	0.00	2.6 ± 0.5	1.2 ± 0.4	0.003	0.001

CG: control group; SG: sepsis group; TG: treatment group; p^a: comparison of CG and SG; p^b: comparison of SG and TG.

Figure 1. Light microscopy of brain tissues in different groups [hematoxylin and eosin (H&E), magnification 200×]. (a) Normal cerebral cortex (control group). (b) Pyknosis, degeneration at the capillary vessels, perineural and perivascular edema (sepsis group). (c) perineural and perivascular mild/moderate edema (treatment group).