Effects of fullerene C60 on liver tissue in liver ischemia reperfusion injury in rats undergoing sevoflurane anesthesia

Figures & data

Figure 1. Light microscopic view of liver tissue of group C; normal liver tissue (HL: hepatic lobules; VC: vena centralis; k: kupffer cell hyperplasia; *: sinusoid dilatation; ↓↓: infiltration; →: hepatocyte; c: dikaryotic hepatocytes; e: erythrocyte), H&EX100.

Figure 2. Light microscopic view of liver tissue of group FC60; normal liver tissue (HL: hepatic lobules; VC: vena centralis; e: erythrocyte; con: congestion; *: sinusoid dilatation; →: hepatocyte; c: dikaryotic hepatocytes; ↓↓: infiltration), H&EX100.

Figure 3. Light microscopic view of liver tissue of group IR (HL: hepatic lobules; VC: vena centralis; e: erythrocyte; con: congestion; *: sinusoid dilatation; ↓↓: infiltration; dikaryotic hepatocytes; inf: inflammation; (*): necrotic and apoptotic appearance in hepatocytes; dej: hydrophilic degeneration), H&EX100.

Figure 4. Light microscopic view of liver tissue of group IR-Sevo (HL: hepatic lobules; VC: vena centralis; *: sinusoid dilatation; ↓↓: infiltration; →: hepatocyte; k: kupffer cell hyperplasia; (*): necrotic and apoptotic appearance in hepatocytes), H&EX100.

Figure 5. Light microscopic view of liver tissue of group IR-FC60 (HL: hepatic lobules; VC: vena centralis; con: congestion; *: sinusoid dilatation; ↓↓: infiltration; →: hepatocyte; c: dikaryotic hepatocytes; k: kupffer cell hyperplasia; dej: hydrophilic degeneration; con: congestion), H&EX100.

Figure 6. Light microscopic view of liver tissue of group IR-FC60-Sevo (HL: hepatic lobules; VC: vena centralis; e: erythrocyte; *: sinusoid dilatation; ↓↓: infiltration; →: hepatocyte; k: kupffer cell hyperplasia; (*): necrotic and apoptotic appearance in hepatocytes), H&EX100.

Figure 7. The comparison of histological changes in rat hepatic tissue [mean ± SD], *p <0.05: when compared with group IR.

Table 1. The comparison of histological changes in rat hepatic tissue [mean ± SD].

Histological Change	Group S (n = 6)	Group FC60 (n = 6)	Group IR (n = 6)	Group IR-Sevo (n = 6)	Group IR-FC60 (n = 6)	Group IR-FC60-Sevo (n = 6)	P**
Hepatocyte degeneration	0.33 ± 0.21*	0.50 ± 0.22*	2.00 ± 0.37	1.00 ± 0.37*	0.50 ± 0.22*	0.83 ± 0.31*	0.004
Sinusoidal dilatation	0.33 ± 0.21*	0.33 ± 0.21*	1.83 ± 0.31	0.83 ± 0.31*	0.67 ± 0.21*	0.83 ± 0.31*	0.004
Pycnotic nucleus	0.33 ± 0.21	0.50 ± 0.22	1.17 ± 0.31	0.67 ± 0.21	0.33 ± 0.21	0.50 ± 0.22	0.147
Prenecrotic cell	0.17 ± 0.17*	0.33 ± 0.21*	1.50 ± 0.22	1.00 ± 0.26	0.50 ± 0.22*	0.83 ± 0.31	0.004
Mononuclear cellular infiltration in the parenchyma	0.33 ± 0.21*	0.33 ± 0.21*	1.67 ± 0.33	1.00 ± 0.26	0.67 ± 0.33*	0.83 ± 0.31*	0.021

p**: Statistical significance was set at a p value < 0.05 for ANOVA test.

*p < 0.05: When compared with Group IR.

Table 2. Oxidative state parameters in rat hepatic tissue [mean ± SD].

	Group S (n = 6)	Group FC60 (n = 6)	Group IR (n = 6)	Group IR-Sevo (n = 6)	Group IR-FC60 (n = 6)	Group IR-FC60-Sevo (n = 6)	P**
TBARS (nmol/mL)	0.40 ± 0.04*	0.48 ± 0.06*	1.45 ± 0.26	0.76 ± 0.17*	0.53 ± 0.07*	0.62 ± 0.08*	<0.0001
CAT (IU/mg.pro)	2.88 ± 0.50*	2.34 ± 0.41*	0.90 ± 0.15	1.60 ± 0.40	2.40 ± 0.37*	1.88 ± 0.37	0.013
GST (IU/mg.pro)	0.67 ± 0.18*	0.53 ± 0.12*	0.06 ± 0.02	0.32 ± 0.12	0.59 ± 0.15*	0.44 ± 0.11*	0.025

p**: Statistical significance was set at a p value < 0.05 for ANOVA test.

*p < 0.05: When compared with Group IR.

Figure 8. Oxidative state parameters in rat hepatic tissue [mean ± SD], *p <0.05: when compared with group IR.