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Original Article

Probiotic Lactobacillus reuteri has antifungal effects on oral Candida species in vitro

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Article: 1274582 | Received 18 Oct 2016, Accepted 16 Dec 2016, Published online: 18 Jan 2017

Figures & data

Figure 1. Co-aggregation ratio (%) between Candida strains and the Lactobacillus reuteri strains after 4 h of incubation. Mean, error bars indicate standard deviations.

*Statistically significant differences (p < 0.05) .L. reuteri DSM 17938: C. tropicalis DSM 7524 showed significantly higher co-aggregation ability compared with C. krusei CCUG 56126, C. dubliniensis 41_3 ZZMK, C. parapsilosis 26 PBS, C. glabrata CBS 863, and C. parapsilosis CCUG 56136. L. reuteri ATCC PTA 5289: C. tropicalis DSM 7524 showed significantly higher co-aggregation ability compared with C. glabrata CBS 863 and C. parapsilosis CCUG 56136. C. krusei CCUG 56126 showed a significantly greater ability to co-aggregate compared with C. parapsilosis CCUG 56136.

Figure 1. Co-aggregation ratio (%) between Candida strains and the Lactobacillus reuteri strains after 4 h of incubation. Mean, error bars indicate standard deviations.*Statistically significant differences (p < 0.05) .L. reuteri DSM 17938: C. tropicalis DSM 7524 showed significantly higher co-aggregation ability compared with C. krusei CCUG 56126, C. dubliniensis 41_3 ZZMK, C. parapsilosis 26 PBS, C. glabrata CBS 863, and C. parapsilosis CCUG 56136. L. reuteri ATCC PTA 5289: C. tropicalis DSM 7524 showed significantly higher co-aggregation ability compared with C. glabrata CBS 863 and C. parapsilosis CCUG 56136. C. krusei CCUG 56126 showed a significantly greater ability to co-aggregate compared with C. parapsilosis CCUG 56136.

Table 1. Growth inhibition of six clinical isolates and six reference Candida strains by two strains of the probiotic Lactobacillus reuteri at different cell concentrations.

Figure 2. Microsensor measurement of pH at selected Candida strains grown by the agar overlay technique with L. reuteri ATCC PTA 5289 (103 cfu/mL) in the bottom agar layer. Zero on the vertical axis represents the first reading when the sensors hit either a Candida colony or the agar plates without Candida colonies. For the Candida controls without lactobacilli, data are only presented for C. krusei CCUG 56126.

Figure 2. Microsensor measurement of pH at selected Candida strains grown by the agar overlay technique with L. reuteri ATCC PTA 5289 (103 cfu/mL) in the bottom agar layer. Zero on the vertical axis represents the first reading when the sensors hit either a Candida colony or the agar plates without Candida colonies. For the Candida controls without lactobacilli, data are only presented for C. krusei CCUG 56126.

Figure 3. Microsensor measurement of pH at selected Candida strains grown by the agar overlay technique with L. reuteri DSM 17938 (103 cfu/mL) in the bottom agar layer. Zero on the vertical axis represents the first reading when the sensors hit either a Candida colony or the agar plates without Candida colonies. For the Candida controls without lactobacilli, data are only presented for C. krusei CCUG 56126.

Figure 3. Microsensor measurement of pH at selected Candida strains grown by the agar overlay technique with L. reuteri DSM 17938 (103 cfu/mL) in the bottom agar layer. Zero on the vertical axis represents the first reading when the sensors hit either a Candida colony or the agar plates without Candida colonies. For the Candida controls without lactobacilli, data are only presented for C. krusei CCUG 56126.