Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania

Figures & data

Figure 1. Villages in Kongwa district showing surveyed households. For the locations of the villages in Kongwa see 5. Black circles, red circles and red stars show soils from households identified as negative, universal ddPCR positive or positive for T. solium, respectively.

Table 1. Primer and probe names and sequences used in ddPCR assay.

Name	Description	Sequence (5ʹ to 3ʹ)	Reference
JB3	Forward	TTTTTTGGGCATCCTGAGGTTTAT	[25]
JB4.5	Reverse	TAAAGAAAGAACATAATGAAAATG	[25]
Tsol_F	Forward	ATTCTTCCGGGGTTTGGTAT	This study
Tsol_R	Reverse	CCTTAATCCCCGTAGGCACT	This study
Tsol_probe	FAM probe	56-FAM/TGTGTTCTGATGCTTTTGGC/31ABkFQ	This study

Table 2. Statistical analysis of mean mitochondrial cox1 gene copies per reaction mixture of T. solium detected both by universal (u) and specific (s) ddPCR assays in DNA samples extracted from household soil tested in duplicate.

Sample	uddPCR	sddPCR	MD	p value	uddPPR			sddPCR
P25	16,946	19,940	2994	< 0.05	Sample pairs	MD	p valuea	MD	p valuea
MA3	41	29	12	> 0.05	MA3 vs MA6	94	> 0.05	107	> 0.05
MA6	135	136	1	> 0.05	MA3 vs ML14	22	> 0.05	24	> 0.05
ML14	63	53	10	> 0.05	MA6 vs ML14	72	> 0.05	83	> 0.05

a Bonferroni’s multiple comparisons test; MD = Mean Difference; MA3 & MA6 and ML14 = Households contaminated by T. solium DNA in Masinyeti and Mlali Iyegu villages, respectively; P25 = Positive control; uddPCR = universal ddPCR assay; sddPCR = specific ddPCR probe assay.

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