Advanced search
Publication Cover
Journal of Extracellular Vesicles Volume 8, 2019 - Issue 1
Journal homepage
2,168
Views
3
CrossRef citations to date
0
Altmetric
Correction

Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by Next-Generation Sequencing

Dominik BuschmannInstitute of Human Genetics, University Hospital, LMU Munich, Germany;Division of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany
,
Benedikt KirchnerDivision of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany;Dr. von Hauner Children’s Hospital, LMU Munich, Germany
,
Stefanie HermannDivision of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany
,
Melanie MärteDepartment of Anesthesiology, University Hospital, LMU Munich, Germany
,
Christine WurmserChair of Animal Breeding, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany
,
Florian BrandesDepartment of Anesthesiology, University Hospital, LMU Munich, Germany
,
Stefan KotschoteIMGM Laboratories GmbH, Planegg, Germany
,
Michael BoninIMGM Laboratories GmbH, Planegg, Germany
,
Ortrud K. SteinleinInstitute of Human Genetics, University Hospital, LMU Munich, Germany
,
Michael W. PfafflDivision of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Germany
,
Gustav SchellingDepartment of Anesthesiology, University Hospital, LMU Munich, Germany
&
Marlene ReithmairInstitute of Human Genetics, University Hospital, LMU Munich, Germany
 show all
Article: 1581487 | Published online: 08 Mar 2019

Figures & data

Figure 7. Morphology of serum EVs by transmission electron microscopy. Images are representative for at least two biological replicates for both volunteers (top panel) and sepsis patients (bottom panel). Scale bars within each panel are 250 nm (top row) and 100 nm (bottom row).

Figure 7. Morphology of serum EVs by transmission electron microscopy. Images are representative for at least two biological replicates for both volunteers (top panel) and sepsis patients (bottom panel). Scale bars within each panel are 250 nm (top row) and 100 nm (bottom row).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related Research Data

Systems biology approaches to investigating the roles of extracellular vesicles in human diseases.
Source: Springer Science and Business Media LLC
Incidence of severe sepsis and septic shock in German intensive care units: the prospective, multicentre INSEP study
Source: Springer Science and Business Media LLC
Neuronal-derived extracellular vesicles are enriched in the brain and serum of HIV-1 transgenic rats.
Source: Informa UK Limited
Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma
Source: Springer Science and Business Media LLC
ggplot2
Source: Springer New York
Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility.
Source: Informa UK Limited
Nanoparticle tracking analysis of microvesicles and exosomes
Source: Wiley
Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.
Source: HAL CCSD
Size-dependent cellular uptake of exosomes
Source: Elsevier BV
RNAcentral: an international database of ncRNA sequences.
Source: Oxford University Press (OUP)
Importance of secreted bacterial RNA in bacterial-host interactions in the gut.
Source: Elsevier BV
Serum miR-122 levels are related to coagulation disorders in sepsis patients
Source: Walter de Gruyter GmbH
ggfortify: Unified Interface to Visualize Statistical Results of Popular R Packages
Source: The R Foundation
Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery
Source: Informa UK Limited
Optimized exosome isolation protocol for cell culture supernatant and human plasma
Source: Informa UK Limited
Comparative miRNA Analysis of Urine Extracellular Vesicles Isolated through Five Different Methods.
Source: MDPI AG
Analysis of Extracellular Vesicles in Gastric Juice from Gastric Cancer Patients
Source: MDPI AG
Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine
Source: Elsevier BV
Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma
Source: Wiley
Evaluation of circulating small extracellular vesicles derived miRNAs as biomarkers of early colon cancer: a comparison with plasma total miRNAs
Source: Informa UK Limited
Importance of RNA isolation methods for analysis of exosomal RNA: evaluation of different methods.
Source: Elsevier BV
Isolation of biologically-active exosomes from human plasma.
Source: Elsevier BV
High-speed centrifugation induces aggregation of extracellular vesicles
Source: Informa UK Limited
MiR-30a targets IL-1α and regulates islet functions as an inflammation buffer and response factor
Source: Springer Science and Business Media LLC
Small non-coding RNA landscape of extracellular vesicles from human stem cells.
Source: Springer Science and Business Media LLC
Serum miR-122 correlates with short-term mortality in sepsis patients
Source: Springer Science and Business Media LLC
The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling
Source: Informa UK Limited
Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR
Source: Springer New York
Different EV enrichment methods suitable for clinical settings yield different subpopulations of urinary extracellular vesicles from human samples
Source: Taylor & Francis Group
A new method of classifying prognostic comorbidity in longitudinal studies: Development and validation☆
Source: Elsevier BV
The role of extracellular vesicles in biomineralisation: current perspective and application in regenerative medicine.
Source: SAGE Publications
Serum microRNA signatures identified by Solexa sequencing predict sepsis patients mortality: A prospective observational study
Source: Public Library of Science (PLoS)
Coagulation dysfunction in sepsis and multiple organ system failure.
Source: Elsevier BV
Charge-based precipitation of extracellular vesicles
Source: Spandidos Publications
Characterization of human plasma-derived exosomal RNAs by deep sequencing
Source: Springer Science and Business Media LLC
Levels of circulating miR-133a are elevated in sepsis and predict mortality in critically ill patients.
Source: Ovid Technologies (Wolters Kluwer Health)
Cellular and extracellular miRNAs are blood-compartment-specific diagnostic targets in sepsis.
Source: Ludwig-Maximilians-Universität München
Integrated isolation and quantitative analysis of exosome shuttled proteins and nucleic acids using immunocapture approaches.
Source: Elsevier BV
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
Source: Springer Science and Business Media LLC
A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents
Source: Public Library of Science (PLoS)
Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection
Source: Springer Science and Business Media LLC

Linking provided by 

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.