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Journal of Extracellular Vesicles Volume 8, 2019 - Issue 1
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Technical Report

Pitfalls associated with lipophilic fluorophore staining of extracellular vesicles for uptake studies

Jens B. SimonsenDepartment of Health Technology (DTU Health Tech) and Department of Micro- and Nanotechnology (DTU Nanotech), Technical University of Denmark, Lyngby, DenmarkCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-4797-8570View further author information
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Article: 1582237 | Received 21 Dec 2018, Accepted 09 Feb 2019, Published online: 20 Feb 2019

Figures & data

Figure 1. Schematic illustration of the proposed paths of the lipid-anchored fluorophore (LAF) used for labelling and tracking EVs that are discussed in this commentary.

(a) LAF labelling of non-EV serum components, that are often present in EV isolates, in addition to EV labelling. (b) LAF labelling of pure EVs. (c) Dissociation of LAFs from EVs into serum components. (d) Formation of LAF particles that exhibit low fluorescence due to self-quenching.

Figure 1. Schematic illustration of the proposed paths of the lipid-anchored fluorophore (LAF) used for labelling and tracking EVs that are discussed in this commentary.(a) LAF labelling of non-EV serum components, that are often present in EV isolates, in addition to EV labelling. (b) LAF labelling of pure EVs. (c) Dissociation of LAFs from EVs into serum components. (d) Formation of LAF particles that exhibit low fluorescence due to self-quenching.

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