Surveillance for Zika in Mexico: naturally infected mosquitoes in urban and semi-urban areas

ABSTRACT

Zika cases have been reported in 29 out of the 32 states of Mexico. Information regarding which mosquito species might be driving Zika virus transmission/maintenance in nature must be regularly updated. From January 2017 to November 2018, mosquitoes were collected indoors and outdoors using the CDC backpack aspirator in urban and semi-urban areas with evidence of mosquito-borne disease transmission. 3873 mosquito pools were tested for Zika infection using the CDC Trioplex real-time RT-PCR. For each collected specie, maximum likelihood estimator of infection rate (MLE) was estimated. Results showed 492 mosquito pools positive for Zika virus RNA. The majority of the positive pools were Aedes (Stegomyia) aegypti (Linnaeus) (54.6%, MLE = 19) (males and females) and Culex (Culex) quinquefasciatus (Say) (19.5%, MLE = 16.8). For the first time, ZIKV infection was detected in Ae. (Georgecraigius) epactius (Dyar and Knab) (MLE = 17.1), Cx. (Melanoconion) erraticus (Dyar and Knab) (MLE = non-estimable), Culiseta (Culiseta) inornata (Williston) (MLE = non estimable), and Cs (Cs.) particeps (Adams) (MLE = 369.5). Other detected species were: Ae. (Stg.) albopictus (Skuse) (MLE = 90.5), Cx. (Cx.) coronator s.l. (Dyar and Knab) (MLE = 102.8) and Cx. (Cx.) tarsalis (Coquillett) (MLE = 117.2). However, our results do not allow for the incrimination of these species as vectors of ZIKV. Routine surveillance should start to consider other mosquito species across the taxonomic spectrum of the Culicidae.

KEYWORDS:

• Arbovirus
• Culicidae
• mosquito-borne diseases
• vector control

Acknowledgments

We would like to thank all the staff from each of the Mexican Specialized Bioassay Units of in the surveyed states for their assistance with field work. We are grateful to all the staff of in the Department of Virology InDRE and to the Red Nacional de Laboratorios de Salud Publica (RNLSP) for molecular work. Luis M. Hernández-Triana would like to thank the EU Framework Horizon 2020 Innovation Grant, European Virus Archive (EVAg, grant no. 653316) and the UK Department for Environment and Rural Affairs (DEFRA), Scottish Government and Welsh Government (grant nos. SV3045, SE4113) for travel expenses. We thank Selene Garcia-Luna for assisting us with the MLE analysis. We are extremely grateful to Audrey Lenhart for her comments, suggestions and proofreading this paper for the use of English. We thank two anonymous reviewers for invaluable comments.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

Supplemental data for this article can be accessed here.