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Research Article

Circulation of dengue serotype 1 viruses during the 2019 outbreak in Dar es Salaam, Tanzania

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Figures & data

Figure 1. Sample flow and experimental approach used in this study. The chart illustrates the methods for screening non-structural protein 1 (NS1) and anti-DENV immunoglobulin G (IgG) and IgM using a commercial OnSite Duo Dengue Ag-IgG/IgM rapid test (CTK BIOTECH Inc, CA, USA), detection of DENV RNA by conventional reverse transcription polymerase chain reaction (RT-PCR) and genetic characterization after sequencing and phylogenetic analysis

Figure 1. Sample flow and experimental approach used in this study. The chart illustrates the methods for screening non-structural protein 1 (NS1) and anti-DENV immunoglobulin G (IgG) and IgM using a commercial OnSite Duo Dengue Ag-IgG/IgM rapid test (CTK BIOTECH Inc, CA, USA), detection of DENV RNA by conventional reverse transcription polymerase chain reaction (RT-PCR) and genetic characterization after sequencing and phylogenetic analysis

Table 1. List of primers used in conventional RT-PCR

Table 2. Socio-demographic characteristics of the participants and serological results

Table 3. The characteristics of RT-PCR positive sera samples

Table 4. Representative DENV virus isolates/strains used for reconstruction of phylogenetic tree

Table 5. Basic local alignment search results for homologous DENV-1 sequences available at GenBank database

Figure 2. Phylogenetic tree of DENV partial polyprotein gene sequences at capsid pre-membrane junction region (CprM). DENV-1 strains detected during the 2019 outbreak in Tanzania are indicated in black squares. The evolutionary relationship was inferred by the Maximum likelihood method in 1000 bootstrap replicates. The bootstrap support values >80% are shown at the nodes. The scale bar indicates nucleotide substitutions per site

Figure 2. Phylogenetic tree of DENV partial polyprotein gene sequences at capsid pre-membrane junction region (CprM). DENV-1 strains detected during the 2019 outbreak in Tanzania are indicated in black squares. The evolutionary relationship was inferred by the Maximum likelihood method in 1000 bootstrap replicates. The bootstrap support values >80% are shown at the nodes. The scale bar indicates nucleotide substitutions per site