Molecular identification of the potentially forensically relevant cluster flies Pollenia rudis (Fabricius) and Pollenia vagabunda (Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

Figures & data

Table 1. List of species used for genetic analysis with their GenBank accession numbers and/or BOLD sequence ID.

Species	GenBank accession No.	BOLD sequence ID	Country
Pollenia amentaria (Scopoli, 1763)	GQ409350.1	–	Unknown
	–	GMGMG197-14	Germany
Pollenia angustigena Wainwright, 1940	KY749783	GBMIN53895-17	Poland
Pollenia griseotomentosa (Jacentkovský, 1944)	JF867619	BBDCN031-10	Canada
	–	GMGMB1441-14	Germany
	KR743688	SMTPB3144-13	Canada
Pollenia hungarica Rognes, 1987	–	GMGMH527-14	Germany
	MK883262	–	China
Pollenia labialis Robineau-Desvoidy, 1863	MG755746	–	China
	HM883114	BBDED147-10	Canada
	JQ807043.1	–	USA
Pollenia leclercqiana Lehrer, 1978	KY860000	–	Portugal
Pollenia pediculata Macquart, 1834	MG118651	AGAKR005-17	Canada
	–	GMEGA657-14	Egypt
	–	GBMWB1680-15	Germany
	KT368668	GBMIN53897-17	Ireland
	MG673561	GBDP30823-19	Norway
	KY749784	GBMIN53899-17	Poland
	JQ807044	–	USA
Pollenia rudis Fabricius, 1794	MT299918	DZPO001-20	Algeria
	MT299919	DZPO002-20	Algeria
	MT299920	DZPO003-20	Algeria
	MT299921	DZPO004-20	Algeria
	MT299922	DZPO005-20	Algeria
	MT299923	DZPO006-20	Algeria
	FR719179	GBMIN30477-13	UK
	JQ807056	–	USA
	MG121409	ASDMT856-11	Canada
	KT368666	GBMIN53901-17	Ireland
	KY749785	GBDP30488-19	Poland
	–	GBMWB1072-15	Germany
Pollenia vagabunda (Meigen, 1826)	MT309586	DZPO007-20	Algeria
	MT309587	DZPO008-20	Algeria
	MT309588	DZPO009-20	Algeria
	MT309589	DZPO010-20	Algeria
	MT309590	DZPO011-20	Algeria
	MT309592	DZPO012-20	Algeria
	–	GMGMG088-14	Germany
	MK883263	–	China
	MG120403	ASDMT1354-11	Canada

Table 2. Absolute abundance of adult Pollenia rudis and Pollenia vagabunda according to the decomposition stages of rabbit carcasses.

	Decomposition stage
Species	Fresh	Bloated	Decay	Dry	Total
Pollenia rudis	0	0	30	127	157
Pollenia vagabunda	0	42	47	236	325

Table 3. Intra- and interspecific P-distances of the analysed cytochrome c oxidase I gene (COI) expressed as percentages.

No.	Species	Intra.	1	2	3	4	5	6	7	8	9	10	11
1	P. rudis DZ	0
2	P. vagabunda DZ	0–0.33	9.59
3	P. rudis W	0	0.66	10.25
4	P. vagabunda W	0	9.87	0.71	9.87
5	P. pediculata W	0–0.66	3.10	8.42	3.76	8.69
6	P. amentaria W	0.66	8.55	8.61	9.21	8.88	8.04
7	P. hungarica W	0.30	3.78	8.94	4.44	9.21	2.94	9.87
8	P. leclercqiana PT	–	8.22	10.91	8.88	11.18	8.18	7.89	9.87
9	P. labialis W	0–0.33	8.22	8.50	8.22	8.44	8.36	9.10	8.55	9.43
10	P. griseotomentosa W	0	9.21	8.94	9.87	9.21	8.36	8.55	8.55	9.87	7.79
11	P. angustigena W	0.33	5.43	8.44	6.09	8.72	7.18	9.38	5.76	8.72	7.62	6.74
12	P. alaskensis	–	9.54	11.24	10.20	11.84	9.35	7.24	10.69	10.20	9.87	9.87	11.02

DZ: Algeria; Intra.: intraspecific divergence; PT: Portugal; W: world.

Figure 1. Maximum parsimony consensus phylogram of 41 cytochrome c oxidase I (COI) sequences from nine Pollenia species and one outgroup (Calliphora alaskensis) based on 10 000 bootstrap replicates.