Determination of 3-MeO-PCP in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification

Figures & data

Figure 1. 3-Methoxyphencyclidine (3-MeO-PCP) chemical structure.

Figure 2. Plastic bag containing 3-methoxyphencyclidine (3-MeO-PCP) powder.

Figure 3. Spectral data information for 3-MeO-PCP, and metabolites found in urine of the fatal case, by liquid ­chromatography coupled to high resolution mass spectrometry (LC-HRMS). (A) 3-MeO-PCP (C₁₈H₂₇NO₂; m/z [M + H⁺] 274.2167), (B) O-demethyl-3-MeO-PCP (C₁₇H₂₆NO; m/z [M + H⁺] 260.2012), (C) piperidine-HO-3-MeO-PCP (C₁₈H₂₈NO₂; m/z [M + H⁺] 290.2119), (D) O-demethyl-piperidine-di-HO-3-MeO-PCP (C₁₇H₂₆NO₃; m/z [M + H⁺] 292.2276, (E) piperidine-di-HO-3-MeO-PCP (C₁₈H₂₈NO₃; m/z [M + H⁺] 306.2066), (F) O-demethyl-piperidine-HO-3-MeO-PCP (C₁₇H₂₆NO₂; m/z [M + H⁺] 276.1962) and (G) O-demethyl-cyclohexyl-HO-3-MeO-PCP (C₁₇H₂₆NO₂; m/z [M + H⁺] 276.1961).

Table 1. The ratio (peak area) between 3-methoxyphencyclidine (3-MeO-PCP) detected in blood and urine (525 and 384 ng/mL respectively, 100%) and its metabolites detected.

	Ratio (%)
Metabolites	Blood	Urine
O-demethyl-3-MeO-PCP	17.0	42.0
Piperidine-HO-3-MeO-PCP	8.4	19.0
O-demethyl-piperidine-di-HO-3-MeO-PCP	3.3	7.0
Piperidine-di-HO-3-MeO-PCP	0.1	5.9
O-demethyl-piperidine-HO-3-MeO-PCP	0.9	13.0
O-demethyl-cyclohexyl-HO-3-MeO-PCP	0.1	2.3