Effects of sertraline hydrochloride and fluconazole combinations on Cryptococcus neoformans and Cryptococcus gattii

Figures & data

Table 1. List of strains

Strain	Source	Serotype
92-31	CDC	A
92-28	CDC	A
92-21	CDC	A
92-29	CDC	A
92-2	CDC	A
92-45	CDC	A
92-101	CDC	A
92-13	CDC	A
92-10	CDC	A
92-30	CDC	A
92-1	CDC	A
92-3	CDC	A
92-43	CDC	A
92-56	CDC	A
92-293	CDC	A
H99	Yan et al. 2002	A
92-32	CDC	D
92-134	CDC	D
92-77	CDC	D
JEC20	Yan et al. 2002	D
92-76	CDC	D
92-27	CDC	D
92-198	CDC	D
92-178	CDC	D
92-138	CDC	D
92-170	CDC	D
92-119	CDC	D
92-337	CDC	D
92-47	CDC	AD
92-74	CDC	AD
92-66	CDC	AD
92-46	CDC	AD
92-190	CDC	AD
92-62	CDC	AD
92-5	CDC	AD
92-26	CDC	AD
92-280	CDC	AD
92-283	CDC	AD
92-304	CDC	AD
92-328	CDC	AD
92-354	CDC	AD
F3050	Kidd et al. 2005	B
RB2	Kidd et al. 2005	B
WM178	Kidd et al. 2005	B
A3MR674	Kidd et al. 2005	B
A4M364	Kidd et al. 2005	B
RB28	Kidd et al. 2005	B
WM179	Kidd et al. 2005	B
RM005	Kidd et al. 2005	B
R270	Kidd et al. 2005	B
MC-S-115	Kidd et al. 2005	B
RB67	Kidd et al. 2005	B
92-71	Kidd et al. 2005	B
92-350	Kidd et al. 2005	B
ATCC22019	Hamilton GeneralHospital	Candida parapsilosis
ATCC6258	Hamilton General Hospital	Candida krusei

Figure 1. (a) Linear regression graph showing a strain's susceptibility to fluconazole and its FICI indicate a weak negative correlation. (b) Same as (a) but with a strain's susceptibility to sertraline. Filled circles indicate the MIC versus FICI point for individual strains.

Table 2. Geometric means (range) of the minimum inhibitory concentrations (MIC), fractional inhibitory concentrations (FIC), and fractional inhibitory concentration index (FICI) among the four serotypes of the C. neoformans species complex

Serotype	MIC- Fluconazole (mg/l)	MIC-Sertraline (mg/l)	FIC – Fluconazole (mg/l)	FIC – Sertraline (mg/l)	FICI
A (n = 15)	10.1 (4.0–64.0)	17.9 (10.75–43.0)	2.0 (0.25–32.0)	3.9 (1.34–10.75)	0.473 (0.25–1.0)
D (n =12)	4.1 (2.0–16.0)	20.3 (10.75–43.0)	1.7 (0.25–4.0)	2.5 (1.34–5.38)	0.550 (0.375–0.750)
AD (n =13)	17.8 (4.0–64.0)	19.3 (5.40–43.0)	3.2 (1.0–16.0)	4.3 (1.34–10.75)	0.444 (0.250–0.750)
B (n =13)	16.0 (4.0–64.0)	15.6 (10.75–43.0)	3.4 (1.0–8.0)	3.3 (1.34–10.75)	0.453 (0.250–0.750)

Figure 2. Fluconazole tolerance alone (MIC-FLC) and in the presence of sertraline (FIC-FLC) for each serotype. The serotypes revert to their respective baseline fluconazole susceptibility in the presence of sertraline. The open triangles represent the respective value for individual strains in each category; the filled squares represent the respective mean value for each category with a 95% confidence interval bar.

Yan , Z , Li , X and Xu , J. 2002 . Geographic distribution of mating type alleles of Cryptococcus neoformans in four areas of the United States . J Clin Microbiol. , 40 : 965 – 972 .

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Kidd , S , Guo , H , Bartlett , K , Xu , J and Kronstad , J. 2005 . Comparative gene genealogies indicate that two clonal lineages of Cryptococcus gattii in British Columbia resemble strains from other geographical areas . Eukaryotic Cell , 4 : 1629 – 1638 .

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