Attractions of nematodes to yeasts are influenced by both nematodes and yeasts

Figures & data

Table 1. Strains of yeasts used in this study

Yeast species	Isolate ID	Source	References
Cryptococcus neoformans	JEC20 (VNIV, MATa)	Lab strain	Sun and Xu (2007)
	JEC21 (VNIV, MATα)	Lab strain	Sun and Xu (2007)
	H99 (VNI, MATα)	Human CSF (Cerebrospinal fluid)	Sun and Xu (2007)
	CDC15 (VNI, MATα)	Human CSF	Sun and Xu (2007)
Cryptococcus gattii	A2M R314 (VGI, MATα)	Human, sputum	Kidd et al. (2005)
	A1M R265 (VGII, MATα)	Human, BAL (Bronchoalveolar lavage)	Kidd et al. (2005)
	NIH191 (VGIII, MATa)	Human, CSF	Kidd et al. (2005)
	WM779 (VGIV, MATα)	Cheetah	Kidd et al. (2005)
Candida albicans	CA1	Human bloodstream	Maganti et al. (2011)
	CA4	Human bloodstream	Maganti et al. (2011)
Candida glabrata	CG44	Human bloodstream	Maganti et al. (2011)
Candida parapsilosis	CP3	Human bloodstream	Maganti et al. (2011)
Candida tropicalis	CT9	Human bloodstream	Maganti et al. (2011)
	CT50	Human bloodstream	Maganti et al. (2011)
	E63	Tree hollow in Hamilton, Ontario	Maganti et al. (2012)

Figure 1. A diagram of the attraction assay where nematodes are spread along the blue strip and the yeast is plated in the red L area. Nematode counts are then made 7 hours after exposure in the L and B areas indicated.

Figure 2. Relative attraction abilities of 15 yeast strains for the nematode C. briggsae AF16.

Figure 3. Relative attraction abilities of 15 yeast strains for nematode C. elegans N2.

Table 2. ANOVA analysis of the effect of Cryptococcus yeast strain and nematode species on yeast-attraction abilities to nematodes

Source	Type III sum of squares	df	F	p
Corrected model	0.904	15	8.620	0.000
Nematode species	0.143	1	20.431	0.000
Yeast strain	0.419	7	8.556	0.000
Nematode species × Yeast strain interaction	0.287	7	5.873	0.000
Error	0.594	85
Total	2.524	101
Corrected total	1.498	100

Table 3. ANOVA analysis of the effect of Candida yeast strain and nematode species on yeast-attraction abilities to nematodes

Source	Type III sum of squares	df	F	p
Corrected model	2.436	29	12.219	0.000
Nematode species	0.726	1	105.649	0.000
Yeast strain	0.673	1	6.994	0.000
Nematode species × Yeast strain interaction	0.618	14	6.425	0.000
Error	1.176	14
Total	7.956	171
Corrected total	3.612	201

Table 4. ANOVA analysis of the effect of Candida yeast species and nematode species on yeast-attraction abilities to nematodes

Source	Type III sum of squares	df	F	p
Corrected model	1.452	11	11.554	0.000
Nematode species	0.848	1	74.179	0.000
Yeast species	0.295	5	5.168	0.000
Nematode species × Yeast species interactions	0.216	5	3.786	0.003
Error	2.160	189
Total	7.956	201
Corrected total	3.612	200

Sun S, Xu J. 2007. Genetic analyses of a hybrid cross between serotypes A and D strains of the human pathogenic fungus Cryptococcus neoformans. Genetics. 177:1475–1486. doi:10.1534/genetics.107.078923

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