Species-level assessment of secondary metabolite diversity among Hamigera species and a taxonomic note on the genus

Figures & data

Figure 1. Secondary metabolites from Hamigera sp.

Table 1. Strains of the Hamigera clade examined for secondary metabolites.

Species	Strain number	Origin
H. avellanea	NRRL 1938	Soil, Texas, USA
H. avellanea	NRRL 58017	Soil, Amposta, Spain
Hamigera sp.	NRRL 2108	Rotting guayule, California, USA
H. ingelheimensis	NRRL 3522	Soil, India
H. ingelheimensis	NRRL 6221	Farm soil, Romania
H. ingelheimensis	NRRL 29060	Swine dung, Illinois, USA
H. paravellanea	NRRL 35714	Forest soil, Poland
H. paravellanea	NRRL 35720	Dung, Spain
H. insecticola	NRRL 35442	Coffee berry borer cuticle, Maryland, USA
H. insecticola	NRRL 35443	Coffee berry borer cuticle, Maryland, USA
H. insecticola	NRRL 58093	Indoor air, Indiana, USA
H. inflata	NRRL 58014	Soil, Gran Canary Island, Spain
H. terricola	NRRL 35602	Soil, Costa Rica
H. terricola	NRRL 35717	Soil, French Guiana
H. terricola	NRRL 35719	Soil, Equatorial Guinea
H. pallida	NRRL 35718	Soil, Togo
H. fusca	NRRL 29058	Soil, Australia
H. fusca	NRRL 35601	Soil, Australia
H. fusca	NRRL 35721	Soil, Grande Comore, The Comoros

Figure 2. HPLC chromatogram of culture extract of H. insecticola NRRL35442 (shaking culture, A-16 medium, 4 days, HPLC condition A monitored at 254 nm).

Figure 3. HPLC chromatogram of culture extract of H. insecticola NRRL35442 (static culture, A-16 medium, 21 days, HPLC condition A monitored at 254 nm).

Table 2. Distribution of tricinonoic acid and peptide metabolites in Hamigera species.

Species and NRRL strain number	Tricinonoic acid	Avellanins A & B	PF1171B	Avellanin C*	PF1171A	PF1171C	Anthraquinone**
Structural class	Sesquiterpene	Pentapeptides			Hexapeptides		Polyketide
H. avellanea 1938	–	○	○	–	–	–	–
H. avellanea 58017	–	○	○	–	–	–	–
Hamigera sp. 2108	–	○	–	–	–	–	–
H. ingelheimensis 3522	–	–	–	–	–	○	–
H. ingelheimensis 6221	–	–	–	–	–	–	–
H. ingelheimensis 29060	–	–	–	○	–	–	–
H. paravellanea 35714	–	○	○	○	○	○	○
H. paravellanea 35720	–	○	○	–	–	○	–
H. insecticola 35442	○	○	–	–	–	–	–
H. insecticola 35443	○	○	–	–	–	–	–
H. insecticola 58093	○	○	–	–	–	–	–
H. inflata 58014	○	○	–	–	–	–	–
H. terricola 35602	○	○	–	–	–	–	–
H. terricola 35717	○	○	–	–	–	–	–
H. terricola 35719	○	○	–	–	–	–	–
H. pallida 35718	○	○	–	–	–	–	–
H. fusca 29058	○	○	○	–	–	○	–
H. fusca 35601	○	○	○	–	–	○	–
H. fusca 35721	–	–	–	–	–	–	–

Notes: *New compound.

**Aromatic polyketide (UV λ_max = 445 nm); structure not determined.

Figure 4. HPLC chromatogram of culture extract of H. paravellanea NRRL35714 (shaking culture, supermalt, 4 days, HPLC condition B monitored at 254 nm).

Figure 5. HPLC chromatogram of culture extract of H. ingelheimensis NRRL29060 (shaking culture, A-3M, 4 days, HPLC condition B monitored at 254 nm).

Figure 6. Phylogenetic tree showing the relations of Hamigera species.