Botanophila flies, vectors of Epichloë fungal spores, are infected by Wolbachia

Figures & data

Table 1. The number of each fly species found in our total sample and their rates of infection by Wolbachia.

Fly species	N	# Infected	Infected (%)
B. cuspidata	1	1	100
B. dissecta	42	35	83.3
B. laterella	13	6	46.2
B. lobata	12	3	25.0
B. phrenione	6	5	83.3
B. sp 5	4	3	75.0
B. sp. 6	5	5	100
Totals	83	58	69.9

N = sample size of fly larvae.

Table 2. The Wolbachia infection rates of Botanophila flies separated by location, fungus and plant species, and fly species.

Location	GPS	Fungus	Plant	Fly Species	N	# Infected	Infected (%)
Poland	52° 47.397ʹ N 18° 06.064ʹ E	E. typhina	P. distans	B. dissecta	5	2	40.0
				B. laterella	1	1	100.0
				B. cuspidata	1	1	100.0
		E. bromicola	E. repens	B. lobata	4	0	0
	52° 46.544ʹ N 18° 06.190ʹ E	E. typhina	P. distans	B. phrenione	3	3	100.0
				B. laterella	1	1	100.0
	51° 59.29ʹ N 17^o 9.262ʹ W	E. typhina	B. pinnatum	B. dissecta	6	3	50.0
	51^o 54.394ʹ N 17^o 2. 587ʹ W	E. bromicola	B. benekeni	B. lobata	1	1	100.0
				B. laterella	1	0	0
	52^o 46.083ʹ N 17^o 92.444ʹ W	E. typhina	H. mollis	B. dissecta	3	2	66.7
				B. lobata	1	1	100.0
				B. laterlla	1	1	100.0
	52° 27.857ʹ N 16° 55.868ʹ E	E. typhina	D. glomerata	B. dissecta	6	6	100.0
			H. lanatus	B. dissecta	8	8	100.0
			D. glomerata	B. phrenione	2	2	100.0
			D. glomerata	B. laterella	1	1	100.0
			H. lanatus	B. laterella	2	2	100.0
			H. lanatus	B. phrenione	1	0	0
	52^o 15. 277ʹ N 16^o 47.577ʹ W	E. typhina	P. nemoralis	B. dissecta	14	14	100.0
Switzerland	47^o 20.566ʹ N 8^o 37.432ʹ E	E. typhina	B. pinnatum	B. laterella	6	0	0
Oregon (USA)	44° 59.053ʹ N 122° 56.648ʹ W	E. typhina	D. glomerata	B. lobata	2	0	0
Missouri (USA)	40° 14.100ʹ N 92° 41.042ʹ W	E. elymi	E. virginicus	B. sp. 5	3	3	100.0
				B. lobata	4	1	25.0
				B. sp. 6	5	5	100.0
Oklahoma (USA)	36° 7.053ʹ N 97° 6.298ʹ W	E. elymi	E. canadensis	B. sp. 5	1	0	0
TOTAL					83	58	69.9